头颈部癌放疗患者口腔念珠菌毒性变化的体外研究

›› 2015, Vol. 35 ›› Issue (8): 677-680.

头颈部癌放疗患者口腔念珠菌毒性变化的体外研究

张媛媛¹,王宁宁¹,李爱芹²,刘莉娜²,崔继丽²

1. 郑州大学口腔医学院
2. 郑州大学口腔医学院口腔黏膜病学实验室

收稿日期:2014-04-04 修回日期:2014-05-24 出版日期:2015-08-28 发布日期:2015-08-14
通讯作者: 王宁宁 E-mail:yiqizd@163.com

In vitro study on virulence factors of oral candida isolated from head and neck cancer patients receiving radiotherapy

Received:2014-04-04 Revised:2014-05-24 Online:2015-08-28 Published:2015-08-14

摘要/Abstract

摘要： 目的从口腔念珠菌本身毒性变化的角度探讨头颈部癌放疗患者口腔念珠菌的致病机理。方法通过体外实验从磷脂酶活性、溶血性和对颊黏膜上皮细胞的粘附力三方面比较放疗对口腔念珠菌致病性改变的影响。结果放疗后口腔念珠菌致病菌和定植菌与正常口腔念珠菌三者之间，磷脂酶活性和粘附力的差异无统计学意义(P<0.05)；放疗后口腔念珠菌致病菌的溶血性较放疗后口腔念珠菌定植菌及正常口腔念珠菌强，差异有统计学意义(P<0.05)；放疗后口腔念珠菌定植菌与正常口腔念珠菌之间的溶血性改变差异无统计学意义(P<0.05)。结论头颈部癌放疗患者口腔念珠菌的溶血性改变可能是其口腔念珠菌病易感的致病因素之一。

关键词: 头颈部癌, 放射治疗, 口腔念珠菌病, 毒力

Abstract: Objective To study the pathogenesis mechanism of oral candida in patients with head and neck cancer undergoing radiotherapy from the perspective of virulence change.Methods In vitro phospholipase activity, hemolytic activity and adhesion to buccal epithelial cells of oral candida isolated from oral cavities of subjects with head and neck cancer undergoing radiotherapy were measured.Results Oral candida in the candida infection group and the candida colonization group after radiotherapy were detected.The phospholipase activity and adhesion ability to BEC showed no significant difference (P>0.05) in the candida infection,colonization group and the control group after radiotherapy. Secreted hemolysin in the candida infection group after radiotherapy was significantly higher than that of the other two groups (P<0.05) ; secreted hemolysin in the candida colonization group after radiotherapy and the control group showed no significant difference (P>0.05).Conclusions The change of hemolytic activity of oral candidia may be one of the pathogenic factors in head and neck cancer patients who had received radiotherapy.

Key words: head and neck cancer, radiotherapy, oral candidosis, virulence

中图分类号:

R780.2

张媛媛王宁宁李爱芹刘莉娜崔继丽. 头颈部癌放疗患者口腔念珠菌毒性变化的体外研究[J]. 口腔医学, 2015, 35(8): 677-680.

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