富血小板纤维蛋白的研究及在组织修复中的应用

›› 2015, Vol. 35 ›› Issue (8): 691-693.

富血小板纤维蛋白的研究及在组织修复中的应用

许方方¹,刘斌²

1. 第四军医大学口腔医学院口腔颌面外科
2. ：第四军医大学口腔医院军事口腔医学国家重点实验室实验动物中心

收稿日期:2015-02-27 修回日期:2015-04-20 出版日期:2015-08-28 发布日期:2015-08-14
通讯作者: 刘斌 E-mail:kqyljd_liu@126.com
基金资助:
国家自然科学基金

Research of plateletrich fibrin and its application in tissue repair

Received:2015-02-27 Revised:2015-04-20 Online:2015-08-28 Published:2015-08-14

摘要/Abstract

摘要： 富血小板纤维蛋白(PRF)是含有丰富的细胞因子和生长因子等类似天然血凝块的浓缩体。因有助于修复组织缺损，在实验和临床应用研究中得到了广泛的认可。该文就PRF的制备、生理特性、组织修复的应用等研究进展作一综述。

关键词: 富血小板纤维蛋白, 组织缺损, 组织修复

Abstract: Plateletrich fibrin(PRF) is a concentrated source similar to a natural blood clot that contains rich cytokines and growth factors.Due to its function in helping repair tissue defects,PRF has been widely accepted in experimental and clinical studies.This article reviewed the research progress of the preparation of the PRF,its physiological characteristics,and its application in tissue repair.

Key words: platelet-rich fibrin, tissue defect, tissue repair

中图分类号:

Q819

许方方刘斌. 富血小板纤维蛋白的研究及在组织修复中的应用[J]. 口腔医学, 2015, 35(8): 691-693.

参考文献

[1]Assoian RK,Grotendorst GR,Miller DM,et al.Cellular transformation by coordinated action of three peptide growth factors from human platelets[J].Nature,1984,309(5971):804-806
[2]Choukroun J,Adda F,Schoeffler C,et al.UneopportuniteenParoimplantologie le PRF[J].Implantodontie,2001,42:55-62
[3]李龙，赵建辉，刘斌，等.富血小板纤维蛋白体外释放VEGF影响因素的探讨[J].中国美容医学,2012,21(3):427-430
[4]孙洁，张剑明，李彦秋.富血小板纤维蛋白超微结构的观察与探讨[J].口腔医学研究,2010,26(1):98-101
[5]Sam G,Vadakkekuttical RJ,Amol NV.In vitro evaluation of mechanical properties of plateletrich fibrin membrane and scanning electron microscopic examination of its surface characteristics[J].J Indian Soc Periodontol,2015,19(1):32-36
[6]JP3〗Dohan D M,Choukroun J,Diss A,et al.Plateletrich fibrin (PRF):a secondgeneration platelet concentratePart III:leucocyte activation:a new feature for platelet concentrates?[J]..Oral Surg Oral Med Oral Pathol Oral Radiol Endod,2006,101(3):e51-e55
[7]Schar MO,DiazRomero J,Kohl S,et al.Plateletrich concentrates differentially release growth factors and induce cell migration in vitro[J].Clin Orthop Relat Res,2015,473(5):1635-1643
[8]Bouletreau PJ,Warren SM,Spector JA,et al.Factors in the fracture microenvironment induce primary osteoblast angiogenic cytokine production[J].Plast Reconstr Surg,2002,110(1):139-148
[9]Lucarelli E,Beccheroni A,Donati D,et al.Plateletderived growth factors enhance proliferation of human stromal stem cells[J].Biomaterials,2003,24(18):3095-3100
[10]Landesberg R,Roy M,Glickman RS.Quantification of growth factor levels using a simplified method of plateletrich plasma gel preparation[J].J Oral Maxillofac Surg,2000,58(3):297-301
[11]Roberts AB,Sporn MB,Assoian RK,et al.Transforming growth factor type beta:rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro[J].Proc Natl Acad Sci USA,1986,83(12):4167-4171
[12]Cohen S.The stimulation of epidermal proliferation by a specific protein (EGF)[J].Dev Biol,1965,12(3):394-407
[13]Schmidt MB,Chen EH,Lynch SE.A review of the effects of insulinlike growth factor and platelet derived growth factor on in vivo cartilage healing and repair[J].Osteoarthritis Cartilage,2006,14(5):403-412
[14]Chikani V,Reding D,Gunderson P,et al.Wisconsin rural women′s health study psychological factors and blood cholesterol level:difference between normal and overweight rural women[J].Clin Med Res,2004,2(1):47-53
[15]Stoney CM,Bausserman L,Niaura R,et al.Lipid reactivity to stress:IIBiological and behavioral influences[J]..Health Psychol,1999,18(3):251-261
[16]Dohan DM,Choukroun J,Diss A,et al.Plateletrich fibrin (PRF):a secondgeneration platelet concentratePart II:plateletrelated biologic features[J]..Oral Surg Oral Med Oral Pathol Oral Radiol Endod,2006,101(3):e45-e50
[17]Liu B,Tan XY,Liu YP,et al.The adjuvant use of stromal vascular fraction and plateletrich fibrin for autologous adipose t1 transplantation[J].T1 Eng Part C Methods,2013,19(1):1-14
[18]Narang I,Mittal N,Mishra N.A comparative evaluation of the blood clot,plateletrich plasma,and plateletrich fibrin in regeneration of necrotic immature permanent teeth:A clinical study[J].Contemp Clin Dent,2015,6(1):63-68
[19]Mohanty S,Pathak H,Dabas J.Platelet rich fibrin:A new covering material for oral mucosal defects[J].J Oral Biol Craniofac Res,2014,4(2):144-146
[20]Nagaveni NB,Poornima P,Joshi JS,et al.Revascularization of immature,nonvital permanent tooth using plateletrich fibrin in children[J].Pediatr Dent,2015,37(1):1-6
[21]Anitua E,Pelacho B,Prado R,et al.Infiltration of plasma rich in growth factors enhances in vivo angiogenesis and improves reperfusion and t remodeling after severe hind limb ischemia[J].J Control Release,2015,202:31-39
[22]杨勇，杨涛，刘斌，等.自体ADSCs复合PRF修复家兔耳软骨缺损的实验研究[J].现代生物医学进展,2013,13(12):2210-2214
[23]Yuanzheng C,Yan G,Ting L,et al.Enhancement of the repair of dog alveolar cleft by autologous iliac bone,bone marrowderived mesenchymal stem cells and plateletrich fibrin mixture［J/OL］.Plast Reconstr Surg,2015.［2015-04-20］.PMID:25835246.
[24]Mathur A,Bains VK,Gupta V,et al.Evaluation of intrabony defects treated with plateletrich fibrin or autogenous bone graft:A comparative analysis[J].Eur J Dent,2015,9(1):100-108
[25]Zhao JH,Tsai CH,Chang YC.Clinical application of plateletrich fibrin as the sole grafting material in maxillary sinus augmentation［J/OL］.J Formos Med Assoc,2015［2015-04-20］.PMID:25840858