MTA对牙周膜干细胞RANKL/OPG表达水平的影响

›› 2015, Vol. 35 ›› Issue (8): 619-622.

MTA对牙周膜干细胞RANKL/OPG表达水平的影响

周益翔¹,刘根霞¹,马姝²,卢亚蝶¹,张光东¹,于金华³

1. 南京医科大学口腔医学院
2. 江苏省南京医科大学
3. 江苏省口腔医院

收稿日期:2015-02-09 修回日期:2015-05-29 出版日期:2015-08-28 发布日期:2015-08-14
通讯作者: 卢亚蝶 E-mail:luyadie@126.com
基金资助:
国家自然科学基金;江苏省自然科学基金;江苏高校优势学科工程

Effects of MTA on the expression of RANKL and OPG in human periodontal ligament stem cells

Received:2015-02-09 Revised:2015-05-29 Online:2015-08-28 Published:2015-08-14

摘要/Abstract

摘要： 目的探讨MTA对人牙周膜干细胞（periodontal ligament stem cells,PDLSCs）核因子-κB受体活化因子配体（receptor activator of NF-κB ligand,RANKL）、骨保护素（osteoprotegerin,OPG）表达的影响。方法通过酶消化法分离培养PDLSCs，依据碱性磷酸酶（alkaline phosphatase，ALP）活性筛选最佳MTA刺激浓度，将PDLSCs分别于对照组（普通培养基）和2 mg/mL MTA条件培养液培养3 d和7 d后，蛋白免疫印迹法分别检测RANKL、OPG蛋白表达。结果 MTA刺激3 d和7 d组，OPG蛋白表达较对照组升高，RANKL蛋白表达较对照组降低。结论 MTA可通过调节RANKL/OPG系统，参与调节PDLSCs成牙/骨及破牙/骨活性。

关键词: MTA, 人牙周膜干细胞, 核因子-κB受体活化因子配体, 骨保护素

Abstract: Objective To evaluate the effects of MTA on the expression of receptor activator of NFκB ligand (RANKL) and osteoprotegerin (OPG) in human periodontal ligament stem cells (PDLSCs).Methods First,PDLSCs were cultured separately by enzyme digestion.Then,PDLSCs were cultured in αMEM medium supplemented with different concentrations of MTA conditioned medium and the optimal concentration of MTA conditioned medium was determined by alkaline phosphatase (ALP) activity.PDLSCs cultured in αMEM (control group),MTA conditioned medium containing 2 mg/mL MTA for 3 days and 7 days were respectively collected to evaluate the expression of RANKL and OPG at protein level by Western blot.Results For PDLSCs cultured in MTA conditioned medium for 3 days and 7 days,expression of OPG was all higher than that in control group,while expression of RANKL was all lower than that in control group.Conclusions MTA can regulate the odonto/osteogenic and odonto/osteoclastic potential of PDLSCs through RANKL/OPG system.

Key words: mineral trioxide aggregate, periodontal ligament stem cells, receptor activator of NFκB ligand, osteoprotegerin

中图分类号:

R780.1

周益翔刘根霞马姝卢亚蝶张光东于金华. MTA对牙周膜干细胞RANKL/OPG表达水平的影响[J]. 口腔医学, 2015, 35(8): 619-622.

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