钛表面芬母多肽涂层的构建及其抗菌性能探究

doi:10.13591/j.cnki.kqyx.2022.07.002

摘要/Abstract

摘要： 目的制备载芬母多肽(FP)的多孔钛表面并探究其生物相容性及抗菌性能。方法将大颗粒喷砂酸蚀(SLA)钛表面设为对照组,通过碱热和硅烷化处理将FP负载在SLA钛表面,根据FP的不同浓度设置三组实验组,对各组表面进行元素分析;将MC3T3-E1细胞接种于各组样品表面,探究其生物相容性;将金黄色葡萄球菌接种于各组样品表面,检测其抗菌性。结果各实验组均被成功硅烷化,且FP分子在硅烷化的钛表面上附着稳定。与SLA钛表面相比,实验组钛表面具有显著的抗菌性能,5 mg/mL组性能更佳,同时具有良好的生物相容性,能明显上调MC3T3-E1成骨细胞的黏附铺展和增殖。结论载FP的多孔钛表面具有良好的生物相容性和抗菌性。

关键词: 钛, 表面处理, 硅烷化, 多肽, 抗菌性

Abstract: Objective To prepare porous titanium surface loaded with funme peptide (FP) and explore its biocompatibility and antibacterial properties. Methods The sandblast and acid-etching (SLA) titanium was set as the control group, and the FP was loaded on the SLA titanium by alkali heating and silanization treatment. Three experimental groups were set up according to different concentrations of FP, and the surface elements of each group were analyzed. MC3T3-E1 cells were inoculated on the surface of each sample to investigate their biocompatibility. Staphylococcus aureus was inoculated on the surface of each group to detect their antibacterial property. Results All the experimental groups were silanized successfully, and FP molecule adhered stably on the surface of silanized titanium. Compared with SLA titanium, titanium in experimental groups had significant antibacterial performance, and the 5 mg/mL group was better. Meanwhile, it had good biocompatibility and could significantly up-regulate the adhesion and proliferation of MC3T3-E1 cells. Conclusion The porous titanium surface of FP has good biocompatibility and antibacterial property.

Key words: titanium, surface treatment, silanization, polypeptide, antibacterial property

中图分类号:

R783.1

吴奇蓉, 雷晨, 吴迪, 周和阳, 汤春波. 钛表面芬母多肽涂层的构建及其抗菌性能探究[J]. 口腔医学, 2022, 42(7): 582-586.

WU Qirong, LEI Chen, WU Di, ZHOU Heyang, TANG Chunbo. Construction and antibacterial property of funme peptide coating on titanium surface[J]. Stomatology, 2022, 42(7): 582-586.

参考文献

[1] Raabe C,Monje A, Abou-Ayash S, et al. Long-term effectiveness of 6 mm micro-rough implants in various indications: A 4.6- to 18.2-year retrospective study[J]. Clin Oral Implants Res, 2021, 32(8):1008-1018.
[2] Lu XX, Wu ZC, Xu KH, et al. Multifunctional coatings of titanium implants toward promoting osseointegration and preventing infection: Recent developments[J]. Front Bioeng Biotechnol, 2021, 9: 783816.
[3] Kligman S, Ren Z, Chung CH, et al. The impact of dental implant surface modifications on osseointegration and biofilm formation[J]. J Clin Med, 2021, 10(8):1641.
[4] Leng J, He Y, Yuan Z, et al. Enzymatically-degradable hydrogel coatings on titanium for bacterial infection inhibition and enhanced soft tissue compatibility via a self-adaptive strategy[J]. Bioact Mater, 2021, 6(12):4670-4685.
[5] Konopatsky AS, Teplyakova TO, Popova DV, et al. Surface modification and antibacterial properties of superelastic Ti-Zr-based alloys for medical application[J]. Colloids Surf B Biointerfaces, 2022, 209: 112183.
[6] Liu YJ, Wu J, Zhang H, et al. Covalent immobilization of the phytic acid-magnesium layer on titanium improves the osteogenic and antibacterial properties[J]. Colloids Surf B Biointerfaces, 2021, 203: 111768.
[7] Teng W, Zhang ZJ, Wang YK, et al. Iodine immobilized metal-organic framework for NIR-triggered antibacterial therapy on orthopedic implants[J]. Small, 2021, 17(35):e2102315.
[8] Wang S, Yang YM, Li W, et al. Study of the relationship between chlorhexidine-grafted amount and biological performances of micro/nanoporous titanium surfaces[J]. ACS Omega, 2019, 4(19):18370-18380.
[9] Wang D, Haapasalo M, Gao Y, et al. Antibiofilm peptides against biofilms on titanium and hydroxyapatite surfaces[J]. Bioact Mater, 2018, 3(4):418-425.
[10] Haney EF, Barbosa SC,Baquir B, et al. Influence of non-natural cationic amino acids on the biological activity profile of innate defense regulator peptides[J]. J Med Chem, 2019, 62(22):10294-10304.
[11] Wang Y, Zhang JW, Gao T, et al. Covalent immobilization of DJK-5 peptide on porous titanium for enhanced antibacterial effects and restrained inflammatory osteoclastogenesis[J]. Colloids Surf B Biointerfaces, 2021, 202: 111697.
[12] Boix-Lemonche G, Guillem-Marti J, D'Este F, et al. Covalent grafting of titanium with a cathelicidin peptide produces an osteoblast compatible surface with antistaphylococcal activity[J]. Colloids Surf B Biointerfaces, 2020, 185: 110586.
[13] Chen Y, Zhang YF, Wang XH, et al. Antibacterial activity and its mechanisms of a recombinant Funme peptide against Cronobacter sakazakii in powdered infant formula[J]. Food Res Int, 2019, 116: 258-265.
[14] Li WY, Lin F, Hung A, et al. Enhancing proline-rich antimicro-bial peptide action by homodimerization: Influence of bifunctional linker[J]. Chem Sci, 2022, 13(8):2226-2237.
[15] Zhang J, Gong HN, Liao MR, et al. How do terminal modifica-tions of short designed IIKK peptide amphiphiles affect their antifungal activity and biocompatibility?[J]. J Colloid Interface Sci, 2022, 608(Pt 1):193-206.
[16] Li K, Chen J,Xue Y, et al. Polymer brush grafted antimicrobial peptide on hydroxyapatite nanorods for highly effective antibacterial performance[J]. Chem Eng J, 2021, 423: 130133.
[17] Buxadera-Palomero J, Godoy-Gallardo M, Molmeneu M, et al. Antibacterial properties of triethoxysilylpropyl succinic anhydride silane (TESPSA) on titanium dental implants[J]. Polymers, 2020, 12(4):773.
[18] D'Este F, Oro D, Boix-Lemonche G, et al. Evaluation of free or anchored antimicrobial peptides as candidates for the prevention of orthopaedic device-related infections[J]. J Pept Sci, 2017, 23(10):777-789.
[19] Hoyos-Nogués M, Velasco F, Ginebra MP, et al. Regenerating bone via multifunctional coatings: The blending of cell integration and bacterial inhibition properties on the surface of biomaterials[J]. ACS Appl Mater Interfaces, 2017, 9(26):21618-21630.
[20] deZoysa GH, Sarojini V. Feasibility study exploring the potential of novel battacin lipopeptides as antimicrobial coatings[J]. ACS Appl Mater Interfaces, 2017, 9(2):1373-1383.
[21] Fraioli R, Rechenmacher F, Neubauer S, et al. Mimicking bone extracellular matrix: Integrin-binding peptidomimetics enhance osteoblast-like cells adhesion, proliferation, and differentiation on titanium[J]. Colloids Surf B Biointerfaces, 2015, 128: 191-200.
[22] Kawasaki S, Inagaki Y,Akahane M, et al. In vitro osteogenesis of rat bone marrow mesenchymal cells on PEEK disks with heat-fixed apatite by CO₂ laser bonding[J]. BMC Musculoskelet Disord, 2020, 21(1):692.
[23] Masters EA, Hao SP, Kenney HM, et al. Distinctvasculotropic versus osteotropic features of S. agalactiae versus S. aureus implant-associated bone infection in mice[J]. J Orthop Res, 2021, 39(2):389-401.
[24] Edelstein AI, Weiner JA, Cook RW, et al. Intra-articular vancomycin powder eliminates methicillin-resistant S. aureus in a rat model of a contaminated intra-articular implant[J]. J Bone Joint Surg Am, 2017, 99(3):232-238.
[25] Rao H, Choo S, Rajeswari Mahalingam SR, et al. Approaches for mitigating microbial biofilm-related drug resistance: A focus on micro- and nanotechnologies[J]. Molecules, 2021, 26(7):1870.
[26] Xie F, Zan YN, Zhang YL, et al. The cysteine protease ApdS from Streptococcus suis promotes evasion of innate immune defenses by cleaving the antimicrobial peptide cathelicidin LL-37[J]. J Biol Chem, 2019, 294(47):17962-17977.
[27] Wang G, Song QL, Huang S, et al. Effect of antimicrobial peptide microcin J25 on growth performance, immune regulation, and intestinal microbiota in broiler chickens challenged with Escherichia coli and Salmonella[J]. Animals, 2020, 10(2):345.