牙龈卟啉单胞菌第Ⅸ分泌系统组成的研究进展

doi:10.13591/j.cnki.kqyx.2022.12.013

摘要/Abstract

摘要： 第Ⅸ分泌系统(type Ⅸ secretion system,T9SS)至少由16种结构蛋白和4种转录调节因子组成,牙龈卟啉单胞菌通过T9SS将牙龈蛋白酶等毒力因子分泌到外膜并附着于细胞表面。本文介绍了牙龈卟啉单胞菌T9SS结构蛋白及其转录调节因子的最新研究进展,进一步理解T9SS跨外膜转运蛋白装置的组成及功能。

关键词: 牙龈卟啉单胞菌, 第Ⅸ分泌系统, 结构蛋白, 转录调节因子

Abstract: The type Ⅸ secretion system(T9SS) consists of at least 16 potential structural proteins and 4 transcription regulators. Porphyromonas gingivalis utilizes T9SS to transport many virulence factors, such as gingipain, across the outer membrane and attach them to the cell surface. This review focusses on the recent advances made in structural proteins and transcription regulators on the T9SS of Porphyromonas gingivalis and to provide further insight into the composition and function of T9SS translocon.

Key words: Porphyromonas gingivalis, type Ⅸ secretion system, structural protein, transcription regulators

中图分类号:

R781.4

李碧榕, 蔡青, 尹昭懿, 王子璇, 孟维艳. 牙龈卟啉单胞菌第Ⅸ分泌系统组成的研究进展[J]. 口腔医学, 2022, 42(12): 1118-1122.

LI Birong, CAI Qing, YIN Zhaoyi, WANG Zixuan, MENG Weiyan. Progress of research on composition of the Ⅸ secretory system of Porphyromonas gingivalis[J]. Stomatology, 2022, 42(12): 1118-1122.

参考文献

[1] Slots J. Periodontitis: Facts, fallacies and the future[J]. Periodontol 2000, 2017, 75(1):7-23.
[2] Lamont RJ, Koo H, Hajishengallis G. The oral microbiota: Dynamic communities and host interactions[J]. Nat Rev Microbiol, 2018, 16(12):745-759.
[3] Benedyk M, Marczyk A,Chruścicka B. Type Ⅸ secretion system is pivotal for expression of gingipain-associated virulence of Porphyromonas gingivalis[J]. Mol Oral Microbiol, 2019, 34(6):237-244.
[4] Shoji M, Nakayama K. Glycobiology of the oral pathogen Porphyromonas gingivalis and related species[J]. Microb Pathog, 2016, 94: 35-41.
[5] Xu WZ, Zhou W, Wang HZ, et al. Roles of Porphyromonas gingivalis and its virulence factors in periodontitis[J]. Adv Protein Chem Struct Biol, 2020, 120: 45-84.
[6] Li N, Zhu YT, LaFrentz BR, et al. The type Ⅸ secretion system is required for virulence of the fish pathogen Flavobacterium columnare[J]. Appl Environ Microbiol, 2017, 83(23):e01769-17.
[7] Veith PD, Glew MD, Gorasia DG, et al. Type Ⅸ secretion: The generation of bacterial cell surface coatings involved in virulence, gliding motility and the degradation of complex biopolymers[J]. Mol Microbiol, 2017, 106(1):35-53.
[8] Gorasia DG, Veith PD, Reynolds EC. The type Ⅸ secretion system: Advances in structure, function and organisation[J]. Microorganisms, 2020, 8(8):1173.
[9] Veillard F, Sztukowska M, Nowakowska Z,et al. Proteolytic processing and activation of gingipain zymogens secreted by T9SS of Porphyromonas gingivalis[J]. Biochimie, 2019, 166:161-172.
[10] Gorasia DG, Glew MD, Veith PD, et al. Quantitative proteomic analysis of the type Ⅸ secretion system mutants in Porphyromonas gingivalis[J]. Mol Oral Microbiol, 2020, 35(2):78-84.
[11] Emrizal R, Nor Muhammad NA. Identification of sequence motifs for the protein components of type Ⅸ secretion system[J]. SainsMalays, 2018, 47(12):2941-2950.
[12] Veith PD, Gorasia DG, Reynolds EC. Towards defining the outer membrane proteome of Porphyromonas gingivalis[J]. Mol Oral Microbiol, 2021, 36(1):25-36.
[13] Glew MD, Veith PD, Peng B, et al. PG0026 is the C-terminal signal peptidase of a novel secretion system of Porphyromonas gingivalis[J]. J Biol Chem, 2012, 287(29):24605-24617.
[14] Gorasia DG, Veith PD, Hanssen EG, et al. Structural insights into the PorK and PorNcomponents of the Porphyromonas gingivalis type Ⅸ secretion system[J]. PLoS Pathog, 2016, 12(8):e1005820.
[15] Heath JE, Seers CA, Veith PD, et al. PG1058 is a novel multidomain protein component of the bacterial type Ⅸ secretion system[J]. PLoS One, 2016, 11(10):e0164313.
[16] Lasica AM, Goulas T, Mizgalska D, et al. Structural and functional probing of PorZ, an essential bacterial surface component of the type-Ⅸ secretion system of human oral-microbiomic Porphyromonas gingivalis[J]. Sci Rep, 2016, 6: 37708.
[17] Glew MD, Veith PD, Chen DN, et al. PorV is an outer membrane shuttle protein for the type Ⅸ secretion system[J]. Sci Rep, 2017, 7(1):8790.
[18] Vincent MS, Canestrari MJ, Leone P, et al. Characterization of the Porphyromonas gingivalis type Ⅸ secretion trans-envelope PorKLMNP core complex[J]. J Biol Chem, 2017, 292(8):3252-3261.
[19] Dou Y, Rutanhira H, Chen X, et al. Role of extracytoplasmic function Sigma factor PG1660 (RpoE) in the oxidative stress resistance regulatory network of Porphyromonas gingivalis[J]. Mol Oral Microbiol, 2018, 33(1):89-104.
[20] Lauber F, Deme JC, Lea SM, et al. Type 9 secretion system structures reveal a new protein transport mechanism[J]. Nature, 2018, 564(7734):77-82.
[21] Leone P, Roche J, Vincent MS, et al. Type Ⅸ secretion system PorM and gliding machinery GldM form Arches spanning the periplasmic space[J]. Nat Commun, 2018, 9(1):429.
[22] Sato K, Okada K, Nakayama K, et al. PorM, a core component of bacterial type Ⅸ secretion system, forms a dimer with a unique kinked-rod shape[J]. Biochem Biophys Res Commun, 2020, 532(1):114-119.
[23] Trinh NTT, Tran HQ, Dong QV, et al. Crystal structure of Type IX secretion system PorE C-terminal domain from Porphyromonas gingivalis in complex with a peptidoglycan fragment[J]. Sci Rep, 2020, 10(1):7384.
[24] Lasica AM, Ksiazek M, Madej M, et al. The type Ⅸ secretion system (T9SS):Highlights and recent insights into its structure and function[J]. Front Cell Infect Microbiol, 2017, 7: 215.
[25] de Diego I, Ksiazek M, Mizgalska D, et al. The outer-membrane export signal of Porphyromonas gingivalistype IX secretion system (T9SS) is a conserved C-terminal β-sandwich domain[J]. Sci Rep, 2016, 6: 23123.
[26] Veith PD, Shoji M, O'Hair RAJ, et al. Type IX secretion system cargo proteins are glycosylated at the C Terminus with a novel linking sugar of the wbp/vim pathway[J]. mBio, 2020, 11(5):e01497-e01420.
[27] Kulkarni SS, Zhu YT, Brendel CJ, et al. Diverse C-terminal sequences involved in Flavobacterium johnsoniaeprotein secretion[J]. J Bacteriol, 2017, 199(12):e00884-e00816.
[28] Kadowaki T, Yukitake H, Naito M,et al. A two-component system regulates gene expression of the type Ⅸ secretion component proteins via an ECF Sigma factor[J]. Sci Rep, 2016, 6: 23288.
[29] Yukitake H, Shoji M, Sato K,et al. PorA, a conserved C-terminal domain-containing protein, impacts the PorXY-SigP signaling of the type Ⅸ secretion system[J]. Sci Rep, 2020, 10(1):21109.
[30] Sato K, Naito M, Yukitake H,et al. A protein secretion system linked to bacteroidete gliding motility and pathogenesis[J]. Proc Natl Acad Sci USA, 2010, 107(1):276-281.
[31] Naito M, Tominaga T, Shoji M,et al. PGN₀₂₉₇ is an essential component of the type Ⅸ secretion system (T9SS) in Porphyromonas gingivalis: Tn-seq analysis for exhaustive identification of T9SS-related genes[J]. MicrobiolImmunol, 2019, 63(1):11-20.
[32] Ono S, Nakayama M, Tachibana M,et al. Construction and characterization of a PGN₀₂₉₇mutant of Porphyromonas gingivalis: Evidence of the contribution of PGN₀₂₉₇ to gingipain activity[J]. Acta Med Okayama, 2019, 73(4):315-323.
[33] Hennell James R, Deme JC, Kjær A, et al. Structure and mechanism of the proton-driven motor that powers type 9 secretion and gliding motility[J]. Nat Microbiol, 2021, 6(2):221-233.
[34] Taguchi Y, Sato K, Yukitake H,et al. Involvement of an skp-like protein, PGN₀₃₀₀, in the type Ⅸ secretion system of Porphyromonas gingivalis[J]. Infect Immun, 2015, 84(1):230-240.
[35] Mizgalska D, Goulas T,Rodríguez-Banqueri A, et al. Intermolecular latency regulates the essential C-terminal signal peptidase and sortase of the Porphyromonas gingivalis type-IX secretion system[J]. Proc Natl Acad Sci USA, 2021, 118(40):e2103573118.
[36] Madej M, Nowakowska Z, Ksiazek M,et al. PorZ, an essential component of the type Ⅸ secretion system of Porphyromonas gingivalis, delivers anionic lipopolysaccharide to the PorUsortase for transpeptidase processing of T9SS cargo proteins[J]. mBio, 2021, 12(1):e02262-e02220.
[37] Park JS, Lee WC, Yeo KJ, et al. Mechanism of anchoring of OmpA protein to the cell wall peptidoglycan of the gram-negative bacterial outer membrane[J]. FASEB J, 2012, 26(1):219-228.
[38] Vincent MS, Durand E, Cascales E. The PorXresponse regulator of the Porphyromonas gingivalis PorXY two-component system does not directly regulate the type Ⅸ secretion genes but binds the PorLsubunit[J]. Front Cell Infect Microbiol, 2016, 6: 96.
[39] Yang DZ, Jiang CZ, Ning B, et al. The PorX/PorY system is a virulence factor of Porphyromonas gingivalis and mediates the activation of the type Ⅸ secretion system[J]. J Biol Chem, 2021, 296: 100574.
[40] Dou Y, Aruni W, Muthiah A,et al. Studies of the extracytoplasmic function Sigma factor PG0162 in Porphyromonas gingivalis[J]. Mol Oral Microbiol, 2016, 31(3):270-283.
[41] Fujise K, Kikuchi Y, Kokubu E,et al. Effect of extracytoplasmic function Sigma factors on autoaggregation, hemagglutination, and cell surface properties of Porphyromonas gingivalis[J]. PLoS One, 2017, 12(9):e0185027.
[42] 刘艳群, 李保胜, 李雨阳, 等. DHA对牙龈卟啉单胞菌脂多糖诱导巨噬细胞炎症的抑制作用[J]. 口腔医学, 2021, 41(5):407-412.