三维打印血管的研究进展

doi:10.13591/j.cnki.kqyx.2023.01.014

摘要/Abstract

摘要：

血管化作为骨、牙髓、皮肤等口腔颌面组织再生的关键,一直是组织工程研究的重点。随着三维(three-dimensional, 3D)打印在组织工程领域的发展,构建血管结构的方式逐渐增加。但要达到在形态及功能上高度模拟血管结构的目的,构建精度较高且具备生物功能的高仿真血管结构以促进组织修复仍是3D生物打印需要克服的一大难点。该文总结了三维打印血管结构的新进展,阐述了几种前沿生物制造技术即悬浮打印、同轴打印、4D打印等在构建血管及血管化结构中的应用,并分析和探讨其优缺点与应用前景,为三维打印血管在口腔颌面组织再生修复方面的应用提供参考。

关键词: 3D打印, 血管再生, 悬浮打印, 同轴打印, 4D打印

Abstract:

As the key to regeneration of oral and maxillofacial tissues such as bone, dental pulp and skin, vascularization has always been the focus of tissue engineering. With the development of three-dimensional (3D) printing in tissue engineering, there are increasing ways to construct vascular structures. However, to achieve the objective of highly simulating vascular structure in morphology and function and promote tissue repair, it is still a major difficulty for 3D bioprinting to construct highly precise and biologically functional simulated vascular structures. This paper summarizes new progress of 3D printing vascular structure, expounds frontier biological manufacturing technologies of vascular and vascularized structure such as suspension printing, coaxial printing, 4D printing, and so on, analyzes its advantages and disadvantages, and discusses its development prospect, in order to provide reference for the application of 3D printing blood vessels in regeneration and repair of oral and maxillofacial tissues.

Key words: 3D printing, vascularization, suspension printing, coaxial printing, 4D printing

中图分类号:

R783.6

陈艺菲,张辰玥,张璟岚,张滨婧,戎鑫,胡芝爱. 三维打印血管的研究进展[J]. 口腔医学, 2023, 43(1): 82-87.

CHEN Yifei,ZHANG Chenyue,ZHANG Jinglan,ZHANG Binjing,RONG Xin,HU Zhiai. Research advances of 3D printing in vascularization[J]. Stomatology, 2023, 43(1): 82-87.

图/表 3

图1

图2

表1

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作者、发表年	“鞘”材料	“鞘”细胞	“芯”材料	“芯”细胞	内径/μm	壁厚/μm
Turner, 2020^[24]	GelMA	hBMSCs	peptide-CD	HUVECs	400	400
Mao, 2021^[25]	0.3%纯海藻酸钠溶液	大鼠心肌细胞(H9C2) 2×10⁶个/mL	3%鼠尾Ⅰ型胶原 3%氯化钙	HUVECs 2×10⁶个/mL
Shahabipour, 2022^[26]	7%GelMA 2%藻酸盐 1%明胶 100 μg/mL HAp	成骨细胞(MC3T3) 5×10⁶个/mL	5%GelMA 0.5%藻酸盐 2%明胶 100 μg/mL HAp	HUVECs 5×10⁶个/mL	210	210
Taymour, 2021^[27]	9%algMC	人肝细胞癌细胞系(HepG2)	含纤维蛋白和人血浆的9%algMC	小鼠NIH 3T3成纤维细胞系	400	400
Shao, 2020^[21]	5%GelMA	人乳腺细胞系(MDA-MB-231)2×10⁶个/mL或小鼠成骨细胞13细胞系(MC3T3-E1)1×10⁶个/mL	5%明胶	HUVECs 2×10⁶个/mL	等效直径 200~1 000