Abstract: Abstract: Objective To investigate the effects of Puerarin with different concentrations on inducing osteogenic differentiation of bone marrowmesenchymal stem cells （BMSCs）,and to explore the relationship between the mechanism of Puerarins osteoinductivity and the Notch signaling pathway in vitro.Methods There divided 6 groups according to the concentration of Puerarin.After segregated and cultivated for 3 weeks,marrow stromal cells of SD rats in induction group were induced by osteogenesis medium containing different concentrations of Puerarin.In noninduction group,BMSCs were still cultured with DMEM medium.After further cultivation for 2-4 weeks,alkaline phosphatase （ALP）and osteocalcin （OC）were measured to evaluate the in vitro osteoinductivity of Puerarin; Delta like ligand 4 （DLL4）was measured by Elisa kit to determine the relationship between the osteoinductivity and Notch signaling pathway.Results Cells of different concentrations showed morphological and biological characteristics similar to those of osteoblasts.The content of ALP,OC,and DLL4 increased.The increase in the 10-5 mol/L group was more than that of the control group with significant difference and that of other groups of different concentrations with no statistical significance.Conclusions Puerarin can induce BMSCs to differentiate into osteoblasts,and the effect is the best with the concentration of 10-5 mol/L.Meanwhile,its mechanism may be related with Notch signaling pathway.

Key words: implant denture , osteogenic induction