›› 2015, Vol. 35 ›› Issue (12): 1008-1012.

• Basic Research • Previous Articles     Next Articles

The study of PDGFRα gene expression in the C57BL/6J mouse embryo palate shelves

  

  • Received:2015-06-23 Revised:2015-08-17 Online:2015-12-28 Published:2015-12-15

Abstract: Objective To observe the expression of PDGFRα in the development stages of mouse embryo palatal shelves.Methods Palatal shelves of C57BL/6J inbred mouse embryos were obtained on the day of GD13.5,GD14.5 and GD15.5 respectively by using stereoscope microscope.The expression location of PDGFRα protein in the palatal shelf was observed by immunohistochemistry(IHC) staining; and the quantities expression of PDGFRα protein were examined by Western blot(WB) analysis.Results IHC staining showed that PDGFRα was specifically expressed in mouse palatal shelves.In GD13.5,PDGFRα expressed mainly in the palatal epithelium; but in GD14.5,GD15.0,and GD15.5,the PDGFRα expressed both in mesenchymal cells and epithelial cells.The WB analysis showed that proteins expression of PDGFRα in different developmental stages of palatal was consistent with IHC results.In GD14.5,the expression level of PDGFRα reached the peak.The proteins expression in GD13.5,GD15.0 or GD15.5 was significantly different compared with that of in GD14.5.Conclusions The study showed PDGFRα had spatiotemporal expression features during the development stages of mouse embryo palatal shelves.PDGFRα gene may be associated with the development of palatal shelves.

Key words: C57BL/6J mouse, embryo, palatal shelves, PDGFRα

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