›› 2016, Vol. 36 ›› Issue (2): 101-104.

• Basic Research • Previous Articles     Next Articles

The expression and significance of HIF2α in condyle chondrocytes under different stress loadings

  

  • Received:2015-08-28 Revised:2015-11-08 Online:2016-02-28 Published:2016-02-29

Abstract: Abstract: Objective To explore the expression of HIF-2α and its downstream catabolic factors (MMP3, MMP13, ADAMTs-4) in the mandibular condyle chondrocytes (MCC) under different stress loadings. Methods The third generation of MCC were cultured in 5% hypoxia. The uniaxial pressure of 0,1000,2000,4000 units strain (ust) were exerted on the MCC for 2 h. Then the specimens were collected and dyed by trypan blue staining. The morphology and number of cells were observed under a light microscope, and the cell mortality was calculated. Westernblot and RTPCR were performed to detect the changes of expression of HIF-2α and its downstream catabolic factors (MMP3, MMP13, ADAMTs-4). Results With the increase of pressure, the cell mortality increased (P<0.05). The expression of HIF-2α and its downstream catabolic factors (MMP3, MMP13, ADAMTs-4) increased with the increase of pressure (P<0.05). Conclusion The activation of HIF-2α and its downstream catabolic factors by overload pressure may be responsible for the damage of condyle chondrocytes.

Key words: HIF-2α, Catabolic factor, Hypoxia, Stress loading, Condyle chondrocyte

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