Abstract: Objective By comparing the protein composition in the serum of patients with oral squamous cell carcinoma and that in the serum of healthy people, so as to obtain the protein changes in the serum of the patients. Methods Peripheral serum samples of 20 patients with oral squamous cell carcinoma and 20 healthy people were collected. Two-dimensional gel electrophoresis with immobilized pH gradients, mass spectrometry and bioinformatics analysis were employed to screen the differentially expressed proteins in serums of patients with oral squamous cell carcinoma. Results Seven protein spots were significantly differentially expressed in serums of patients with oral squamous cell carcinoma compared with healthy people, and one of them was Chain B, T-To-T(High) Quaternary Transitions In Human Hemoglobin, which was lowly expressed in the serums of patients with oral squamous cell carcinoma.Conclusion Expression of Chain B, T-To-T(High) Quaternary Transitions In Human Hemoglobin: Deshis146beta Deoxy Low-Sal in peripheral serum may play an important role of modulation in the occurrence and development of oral squamous cell carcinoma．

Key words: Oral squamous cell carcinoma(OSCC), Two-dimensional gel electrophoresis(2-DE), Proteome, Mass spectrography analysis