Abstract: Objective Study the expression of β-defensin 4 in original human gingival epithelial cells after BLPS stimulating. Methods In vitro original culture human gingival epithelial cells, HGECs were identified by immunocytochemistry. Subjected to the concentration of BLPS are 10、20、40、80、160ug/ml in HGECs. Test the expression of BD-4 in HGECs by immunocytochemistry and western blotting. Results HGECs grew well. The third generation cells were identified Ck (AE1/AE3) (+)/Vimentin (-). The positive staining granular brown was distributed in cytoplasm, increasing of BLPS concentration expression is more intense by immunocytochemistry. The expression of BD-4 increased with the higher concentration of BLPS by western blotting. The top value of BD-4 protein present in the concentration of BLPS 160ug/ml. There are significant different among the HGECs groups of BLPS stimulated (p< 0.01). Conclusion With the inflammation stimulated increase in human gingival epithelial cells, the expression of BD-4 increased. Prompt the function of BD-4 progress in periodontal disease.

Key words: Indomethacin, BLPS, PDLCs