›› 2016, Vol. 36 ›› Issue (4): 309-313.

• Basic Research • Previous Articles     Next Articles

The effect of shLPR5 on the osseointegration on SLA-treated titanium implant surface

  

  • Received:2015-11-11 Revised:2016-01-11 Online:2016-04-28 Published:2016-04-24
  • Contact: Fang ShiZhao E-mail:muye868@163.com

Abstract: Objective To investigate the effect of shLRP5 on the osseointegration on SLA-treated titanium implant surface by constructing shLRP5 lentivirus vetor. Methods One target gene, LRP5, was testified to be involved in the SLA-treated-peri-implant bone formation. The implants were separated into two groups:LV-LRP5 group and blank group. Twenty implants were inserted into tibias of ten rats. We injected shLRP5 lentivirus vetor around implants of LV-LRP5 group. The effect of the SLA surface on peri-implant bone formation was evaluated by histomorphometric analysis. Bone to implant contact (BIC) and bone area (BA) were measured and statistically analyzed. Results LV-LRP5 transfected BMSCs stably. After 6 weeks of implantation, the implant bone contact rate and bone density in the LV-LRP5 group were lower than those in the blank group. Both two groups had significant difference (P<0.05). Conclusion shLRP5 decreased the osseointegration on SLA-trated titanium implant. The mechanism of shLRP5 that decreased the osseointegration on SLA-trated titanium implant might be the decrease of Wnt/LRP5 pathway or cross talk with other pathways.

Key words: LRP5, SLA treatment, osseointegration

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