外周血来源的间充质干细胞与内皮祖细胞体外构建血管化组织的研究

Abstract

Abstract: Objective To engineer vascularized tissue with two types of cells from peripheral blood. Methods Endothelial progenitor cells (EPC) and peripheral blood-derived mesenchymal stem cells (PBMSC) were isolated from rabbit peripheral blood and identified. Then, they were cultured in fibrin at different ratios under the observation of a microscope until the 7th day after the initial creation. Results EPC and PBMSC were successfully isolated from rabbit peripheral blood. Vascularized tissue were acquired at a certain ratio (EPC were 1 million cells/mL, PBMSC were 2 million cells/mL). Conclusion Certain cells isolated from peripheral blood can be used to acquire vascularized tissue by co-cultured strategy at a certain density in vitro.

Key words: EPC, PBMSC, fibin, vascularization in vitro

CLC Number:

R394.26

References

1. Jayaraman P, Gandhimathi C, Venugopal JR, Becker DL, Ramakrishna S, Srinivasan DK. Controlled release of drugs in electrosprayed nanoparticles for bone tissue engineering. Advanced drug delivery reviews. 2015;94:77-95.
2. Schroeder JE, Mosheiff R. Tissue engineering approaches for bone repair: concepts and evidence. Injury. 2011;42(6):609-13.
3. Carmeliet P, Jain RK. Angiogenesis in cancer and other diseases. Nature. 2000;407(6801):249-57.
4. Rouwkema J, Rivron NC, van Blitterswijk CA. Vascularization in tissue engineering. Trends in biotechnology. 2008;26(8):434-41.
5. Unger RE, Ghanaati S, Orth C, Sartoris A, Barbeck M, Halstenberg S, et al. The rapid anastomosis between prevascularized networks on silk fibroin scaffolds generated in vitro with cocultures of human microvascular endothelial and osteoblast cells and the host vasculature. Biomaterials. 2010;31(27):6959-67.
6. Borges J, Muller MC, Momeni A, Stark GB, Torio-Padron N. In vitro analysis of the interactions between preadipocytes and endothelial cells in a 3D fibrin matrix. Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy. 2007;16(3):141-8.
7. Sun X, Altalhi W, Nunes SS. Vascularization strategies of engineered tissues and their application in cardiac regeneration. Advanced drug delivery reviews. 2015.
8. Lin Y, Weisdorf DJ, Solovey A, Hebbel RP. Origins of circulating endothelial cells and endothelial outgrowth from blood. The Journal of clinical investigation. 2000;105(1):71-7.
9. Ingram DA, Caplice NM, Yoder MC. Unresolved questions, changing definitions, and novel paradigms for defining endothelial progenitor cells. Blood. 2005;106(5):1525-31.
10. Dragoni S, Reforgiato M, Zuccolo E, Poletto V, Lodola F, Ruffinatti FA, et al. Dysregulation of VEGF-induced proangiogenic Ca(2+) oscillations in primary myelofibrosis-derived endothelial colony-forming cells. Experimental hematology. 2015;43(12):1019-30.e3.
11. Fadini GP, Losordo D, Dimmeler S. Critical reevaluation of endothelial progenitor cell phenotypes for therapeutic and diagnostic use. Circulation research. 2012;110(4):624-37.
12. Mena HA, Carestia A, Scotti L, Parborell F, Schattner M, Negrotto S. Extracellular histones reduce survival and angiogenic responses of late outgrowth progenitor and mature endothelial cells. Journal of thrombosis and haemostasis : JTH. 2015.
13. Nieves Torres EC, Yang B, Brahmbhatt A, Mukhopadhyay D, Misra S. Blood outgrowth endothelial cells reduce hypoxia-mediated fibroblast to myofibroblast conversion by decreasing proangiogenic cytokines. Journal of vascular research. 2014;51(6):458-67.
14. Critser PJ, Yoder MC. Endothelial colony-forming cell role in neoangiogenesis and tissue repair. Current opinion in organ transplantation. 2010;15(1):68-72.
15. Li S, Huang KJ, Wu JC, Hu MS, Sanyal M, Hu M, et al. Peripheral blood-derived mesenchymal stem cells: candidate cells responsible for healing critical-sized calvarial bone defects. Stem cells translational medicine. 2015;4(4):359-68.
16. Vidyasekar P, Shyamsunder P, Sahoo SK, Verma RS. Scaffold-free and scaffold-assisted 3D culture enhances differentiation of bone marrow stromal cells. In vitro cellular & developmental biology Animal. 2015.
17. Janeczek Portalska K, Leferink A, Groen N, Fernandes H, Moroni L, van Blitterswijk C, et al. Endothelial differentiation of mesenchymal stromal cells. PloS one. 2012;7(10):e46842.
18. Ng F, Boucher S, Koh S, Sastry KS, Chase L, Lakshmipathy U, et al. PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages. Blood. 2008;112(2):295-307.
19. Chen X, Aledia AS, Ghajar CM, Griffith CK, Putnam AJ, Hughes CC, et al. Prevascularization of a fibrin-based tissue construct accelerates the formation of functional anastomosis with host vasculature. Tissue engineering Part A. 2009;15(6):1363-71.
20. Chen X, Aledia AS, Popson SA, Him L, Hughes CC, George SC. Rapid anastomosis of endothelial progenitor cell-derived vessels with host vasculature is promoted by a high density of cotransplanted fibroblasts. Tissue engineering Part A. 2010;16(2):585-94.

Study of vascularization in vitro with peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments