高脂血症大鼠种植体早期骨结合界面的微观动态分析

Abstract

Abstract: Objective To explore the dynamic change for micro-component and micro-morphology of implant-bone interface at early stage in hyperlipidemic rats. Methods Wistar rats were randomly divided into the experimental group and the control group. Rats of the experimental group were given a high-fat diet to create hyperlipidemia molding rats. After 12 weeks, titanium implants were planted into femora of hyperlipidemic and normal rats. The animals were sacrificed at 1, 3 and 5 days after implantation. Hard tissue sections containing micro-screws were made. The dynamic change for micro-component and micro-morphology of implant-bone interface at early stage were analyzed by energy dispersive X-ray spectrometer (EDS) and scanning electronic microscopy (SEM) respectively. Results There were fewer osteoblasts (P＜0.05) and more osteoclasts (P＜0.05) in the experimental group than in the control group. The ratio of Ca/P of the experimental group was lower than that in the control group (P＜0.05). The content of O in the experimental group was higher than that in the control group (P＜0.05). The content of Ti gradually reduced from implants to bone, and there were no significant differences between the experimental group and the control group. Conclusions The number of osteoblasts and Ca, P deposition of implant-bone interface at early stage in hyperlipidemic rats decreased, while the number of osteoclasts increased. Hyperlipidemia inhibits the early osseointegration of implants.

Key words: implant, scanning electron microscope ( SEM), Energy dispersive X-ray spectrometer (EDS), rats

CLC Number:

R782.1

References

[1]Jung RE,Pjutursson BE,Glauser R,et al.A systematic review of the 5-2008 survival and complication rates of implant-supported single crowns[J].Clin Oral Implants Res,2008,19(2):119-130
[2]Armand K,Ana DVB,GaryK,et al.The Effects of Hyperlipidemia on Implant Osseointegration in the Mouse Femur[J].J Oral Implantol,2015,41(2):e7-e11
[3]高雅，钟晓妮，杨艳红，等.重庆市企事业单位职工血脂水平及分布特征[J].中华心血管病杂志,2012,40(5):432-435
[4]Carroll MD,Kit BK,Lacher DA,et al.Total and high-density lipoprotein cholesterol in adults: National Health and Nutrition Examination Survey,2011-212[J].NCHS Data Brief,2013,10(132):1-8
[5]Choukroun J,Khoury G,Khoury F,et al.Two neglected biologic risk factors in bone grafting and implantology: high low-density lipoprotein cholesterol and low serum vitamin D[J].J Oral Implanto,2014,40(1):110-114
[6]Brodeur MR,Brissette L,Falstrault L,et al.Scavenger receptor of class B expressed by osteoblastic cells are implicated in the uptake of cholesteryl ester and estradiol from LDL and HDL3[J].J Bone Miner Res,2008,23(3):326-337
[7]Luegmayr E,Glantschnig H,Wesolowski GA,etal.Osteoclast formation,survival and morphology are highly dependent on exogenous cholesterol/lipoproteins[J].Cell Death Differ,2004,11(1):108-118
[8]Kourkoumelis N,Balatsoukas I,Tzaphlidou M.Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy[J].J BiolPhys,2012,38(2):279-291
[9]Bodine PV.Wnt signaling control of bone cell apoptosis[J].Cell Res,2008,18(2):248-253
[10]Jian J,Sun L,Cheng X,et al.Calycosin-7-O-β-d-glucopyranosidestimulatesosteoblastdifferentiation through regulating the BMP/WNT signaling pathways[J].Acta Pharm Sin B,2015,5(5):454-460
[11]徐巾诏.高脂血症大鼠种植体周围骨组织中Fizzled受体表达初步探究[D/OL].济南：山东大学，2015[2015-06-11].http://58.194.172.18/Thesis/Thesis/ThesisSearch/Search_DataInit.aspx?dbid=4
[12]Sage AP,Lu J,AttiE,et al.Hyperlipidemia induces resistance to PTH bone anabolism in mice via oxidizedlipids[J].Bone Miner Res,2011,26(6):1197-1206
[13]Mazière C,Salle V,GomilaC,et al.Oxidized low density lipoprotein enhanced RANKL expression in human osteoblast-like cells,Involvement of ERK,NFkappaB and NFAT[J].BiochimBiophysActa,2013,1832(10):1756-1764
[14]Almeida M,Ambrogini E,Han L,et al.Increased lipid oxidation causes oxidative stress,increased peroxisome proliferator-activated receptor-gamma expression,and diminished pro-osteogenicWnt signaling in the skeleton[J].J BiolChem,2009,284(40):27438-27448
[15]Ma HP,Ma XN,GeBF,et al.Icariin attenuates hypoxia-induced oxidative stress and apoptosis in osteoblasts and preserves their osteogenic differentiation potential in vitro[J].Cell Prolif,2014,47(6):527-539
[16]Lu J,Cheng H,AttiE,et al.Role of paraoxonase-1 in bone anabolic effects of parathyroid hormone in hyperlipidemicmice[J].BiochemBiophys Res Commun,2013,431(1):19-24
[17]Li X,Garcia J,Lu J,et al.Roles of parathyroid hormone (PTH) receptor and reactive oxygen species in hyperlipidemia-induced PTH resistance in preosteoblasts[J].J Cell Biochem,2014,115(1):179-188
[18]Graham LS,Parhami F,TintutY,et al.Oxidized lipids enhance RANKL production by T lymphocytes: implications for lipid-induced bone loss[J].ClinImmunol,2009,133(2):265-275
[19]MariO,MaiN,ChiyomiH,et al.Low-density lipoprotein receptor deficiency causes impaired osteoclastogenesis and increased bone mass in mice because of defect in osteoclastic cell-cell Fusion[J].J Bio Chem,2012,287(23):19229-19241
[20]Marco F,Milena F,Gianluca G,et al.Peri-implant osteogenesis in health and osteoporosis[J].Micron,2005,36(7-8):630-644
[21]Wachi T,Shuto T,Shinohara Y,et al.Release of titanium ions from an implant surface and their effect on cytokine production related to alveolar bone resorption[J].Toxicology,2015,2(327):1-9

Microcosmic and dynamic analysis of the implant-bone interface at early stage in hyperlipidemic rats

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	. Research progress of the effects of hyperlipidemia on osseointegration of titanium implants [J]. , 2019, 39(6): 557-560.
[2]	. Clinical application progress of computer-aided design and computer-aided manufacturing of milled framework in implant fixed restorations for edentulous patients [J]. , 2019, 39(5): 455-458.
[3]	. Research progress of improving the success rate of planting for diabetic patients [J]. , 2019, 39(5): 459-463.
[4]	. The effect of clenched bruxism on the stress distribution of peri-implant bone with distal free-end edentulism: A three-dimensional finite element study [J]. , 2019, 39(5): 409-413.
[5]	. Reflection and summary of postoperative complications of implant-supported fixed restoration in edentulous patients [J]. , 2019, 39(5): 385-389.
[6]	. Contrastive study on alveolar ridge preservation: Tooth extraction through dentoalveolar distraction [J]. , 2019, 39(3): 207-209.
[7]	Shui-Yi Shao. Application and error analysis of implant reference method in measurement of posterior teeth region in the panoramic radiograph [J]. , 2019, 39(3): 214-217.
[8]	. Clinical application and experience of modified internal sinus floor elevation and dental implant in maxilary molar area [J]. , 2019, 39(3): 223-227.
[9]	. Clinical comparative study of PRP combined with allograft bone powder and autogenous iliac bone graft in the treatment of maxillofacial bone defect [J]. , 2019, 39(3): 218-222.
[10]	. Neither open flap debridement nor bone grafting when “saucer-like” marginal bone loss amounts to 3 mm below the implant shoulder after 1-year functional loading [J]. , 2019, 39(2): 164-166.
[11]	. Flap surgery is required when “saucer-like” marginal bone loss amounted to 3 mm below implant shoulder after 1-year loading [J]. , 2019, 39(2): 161-163.
[12]	. Progress research on application of platelet-rich fibrin in dental implantology [J]. , 2019, 39(1): 71-76.
[13]	. Advantages of long-term stability of guided bone regeneration augmentation for alveolar bone reconstruction compared with autogenous bone graft [J]. , 2019, 39(1): 60-62.
[14]	. Study on the maximum width of the micro slit based on the microsphere diffusion method [J]. , 2019, 39(1): 6-9.
[15]	. Research progress of animal models of peri-implantitis [J]. , 2018, 38(8): 747-751.