Axl基因在口腔鳞状细胞癌中的表达及意义

Abstract

Abstract: Objective To investigate the relationship between the expression of Axl gene and oncogenesis of oral squamous cell carcinoma (OSCC). Methods The immunohistochemical expressions of Axl genes in tumor tissues from 42 patients with OSCC (OSCC group) and in correspondingly adjacent tissues (adjacent group) were examined. In vitro, expression of mRNA and protein of Axl in normal human oral epithelium cell (HOEC), immortalized normal oral keratinocyte cell (NOK), tongue squamous cell carcinoma cell lines UM1 and UM2 were detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) and Western blot (WB), respectively. Results There was significant difference between OSCC group and adjacent group (61.9% vs. 26.2%, P＜0.05) in immunohistochemical expression of Axl. Expression of mRNA and proteins of Axl in UM2 and UM1 were significantly higher than those in HOEC and NOK in vitro. Conclusions Strongly expressed in OSCC tissue and cell lineages compared with their normal counterparts, Axl gene may play a role in OSCC oncogenesis.

Key words: Axl, OSCC, expression

CLC Number:

R739.8

References

[1]Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer J Clin,2011,61(2):69-90
[2]Torre L A,Bray F,Siegel RL,et al.Global cancer statistics,2012[J].CA Cancer J Clin,2015,65(2):87-108
[3]Paccez J D,Vasques GJ,Correa RG,et al.The receptor tyrosine kinase Axl is an essential regulator of prostate cancer proliferation and tumor growth and represents a new therapeutic target[J].Oncogene,2013,32(6):689-698
[4]Hong J,Belkhiri A.AXL mediates TRAIL resistance in esophageal adenocarcinoma[J].Neoplasia,2013,15(3):296-304
[5]Pinato D J,Mauri FA,Lloyd T,et al.The expression of Axl receptor tyrosine kinase influences the tumour phenotype and clinical outcome of patients with malignant pleural mesothelioma[J].Br J Cancer,2013,108(3):621-628
[6]Zhang Z,Lee JC,Lin L,et al.Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer[J].Nat Genet,2012,44(8):852-860
[7]Gustafsson A,Bostrom AK,Ljungberg B,et al.Gas6 and the receptor tyrosine kinase Axl in clear cell renal cell carcinoma[J].PLoS One,2009,4(10):e7575-
[8]Zhao T,Hu F,Qiao B,et al.Telomerase reverse transcriptase potentially promotes the progression of oral squamous cell carcinoma through induction of epithelial-mesenchymal transition[J].Int J Oncol,2015,46(5):2205-2215
[9]O'Bryan JP,Frye RA,Cogswell PC,et al.axl,a transforming gene isolated from primary human myeloid leukemia cells,encodes a novel receptor tyrosine kinase[J].Mol Cell Biol,1991,11(10):5016-5031
[10]Mudduluru G,Allgayer H.The human receptor tyrosine kinase Axl gene--promoter characterization and regulation of constitutive expression by Sp1,Sp3 and CpGmethylation[J].Biosci Rep,2008,28(3):161-176
[11]Mudduluru G,Ceppi P,Kumarswamy R,et al.Regulation of Axl receptor tyrosine kinase expression by miR-34a and miR-199a/b in solid cancer[J].Oncogene,2011,30(25):2888-2899
[12]Paccez J D,Vogelsang M,Parker MI,et al.The receptor tyrosine kinase Axl in cancer: biological functions and therapeutic implications[J].Int J Cancer,2014,134(5):1024-1033
[13]Wu X,Liu X,Koul S,et al.AXL kinase as a novel target for cancer therapy[J].Oncotarget,2014,5(20):9546-9563
[14]Paccez JD,Vasques GJ,Correa RG,et al.The receptor tyrosine kinase Axl is an essential regulator of prostate cancer proliferation and tumor growth and represents a new therapeutic target[J].Oncogene,2013,32(6):689-698
[15]D'Alfonso TM,Hannah J,Chen Z,et al.Axl receptor tyrosine kinase expression in breast cancer[J].J ClinPathol,2014,67(8):690-696
[16]Cichon M A,Szentpetery Z,Caley MP,et al.The receptor tyrosine kinase Axl regulates cell-cell adhesion and stemness in cutaneous squamous cell carcinoma[J].Oncogene,2014,33(32):4185-4192
[17]Nakayama S,Sasaki A,Mese H,et al.Establishment of high and low metastasis cell lines derived from a human tongue squamous cell carcinoma[J].Invasion Metastasis,1998,18(5-6):219-228
[18]Lee CH,Yen CY,Liu SY,et al.Axl is a prognostic marker in oral squamous cell carcinoma[J].Ann SurgOncol,2012,19Suppl 3:500-
[19]Lee CH,Liu SY,Chou KC,et al.Tumor-associated macrophages promote oral cancer progression through activation of the Axl signaling pathway[J].Ann SurgOncol,2014,21(3):1031-1037

Expression and clinical significance of the Axl gene in oral squamous cell carcinoma

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