模拟微重力影响人牙髓干细胞的矿化能力与RhoA-Rho激酶信号通路相关性研究

Abstract

Abstract: Abstract: Objective To investigate the effect of RhoA-Rho kinase signaling pathway on differentiation of human dental pulp stem cells (hDPSCs) in simulated microgravity (SMG). Methods HDPSCs were seeded in the PLGA scaffolds, and divided into normal gravity group and microgravity group. After 72 hours’ culture, cells were seeded in plate and divided randomly into mineralization induced with or without Y-27632 groups. AKPase activity test was established after 3, 5, 7, 10 days. DMP-1 (dentin matrix protein-1), DSP (dentin sialoprotetin) and DSPP (dentin sialophosphoprotein) were detected by Western Blot for 10d, and mineralized nodules were detected by Alizarin red staining for 21d. Results The expression level of RhoA was down-regulated under microgravity. Treated with Y-27632 in normal gravity after 3, 5, 7 and 10 days, the AKPase activity was significantly lower than control. After 21 days’ culture, the Alizarin red staining showed that the positive granules were hardly detected. The expression levels of DSP, DSPP and DMP-1 were lower than those in the control group after 10 days of culture. Conclusion Simulated microgravity inhibited the expression of RhoA. Inactivation of Rho kinase by addition of inhibitor Y-27632 caused lower mineralization ability of hDPSCs. It is possible that RhoA-Rho kinase signaling pathway is involved in the effect of simulated microgravity on mineralization process of hDPSCs.

Key words: human dental pulp stem cells simulated microgravity RhoA-Rho kinase signaling pathway Mineralization

CLC Number:

R780.2

References

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Effects of simulated microgravity on mineralization of hDPSCs via RhoA-Rho signaling pathway

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