壳寡聚糖抑制变形链球菌的致龋能力

Abstract

Abstract: Objective To investigate the effect of chitooligosaccharide (COS) on the growth, acid production and adhesion of streptococcus mutans(S. mutans). Methods Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COS against S. mutans(ATCC 25175) were determined by microdilution technique. The influence of COS on the growth rate of S. mutans was assessed by timekill curve. To measure the impact of COS on acid production of S. mutans, 1 mL bacterial cell suspension containing 1/2 MIC, 1/4 MIC or 1/8 MIC COS was seeded in the sterile culture tubes, and pH value in the collected supernatant was analyzed and pH curve was then figured out. With liquid scintillation counting method, the adverse effect of COS on the bacterial adhesion ability to hydroxylapatite was also fully investigated. Results COS inhibited S. mutans growth with MIC at 2.00 g/L and MBC at 4.00 g/L, respectively. At low concentration, COS exhibited an inhibitory effect on S. mutans growth, while at high concentration, COS was capable of directly killing bacteria. 2.00 g/L (MIC) COS could significantly reduce the acid production and adhesion of S. mutans and this inhibitory effects was in concentrationdependent manners. Conclusion COS can inhibit the growth, acid production and adhesion of S. mutans.

Key words: streptococcus mutans, chitooligosaccharide, growth, acid production, adherence

CLC Number:

R780.1

References

[1]Schwendicke F,Drfer CE,Schlattmann P,et al.Socioeconomic inequality and caries: a systematic review and metaanalysis[J].J Dent Res,2015,94(1):10-18
[2]Kuramitsu HK,Wang BY.The whole is greater than the sum of its parts: dental plaque bacterial interactions can affect the virulence properties of cariogenic Streptococcus mutans[J].Am J Dent,2011,24(3):153-154
[3]Cragg GM,Newman DJ.Natural products: a continuing source of novel drug leads[J].Biochim Biophys Acta,2013,1830(6):3670-3695
[4]Harvey A.The role of natural products in drug discovery and development in the new millennium[J].IDrugs,2010,13(2):70-72
[5]Azuma K,Nishihara M,Shimizu H,et al.Biological adhesive based on carboxymethyl chitin derivatives and chitin nanofibers[J].Biomaterials,2015,42:20-29
[6]Yin H,Du Y,Zhang J.Low molecular weight and oligomeric chitosans and their bioactivities[J].Curr Top Med Chem,2009,9(16):1546-1559
[7]Juiz PJL,Lucchese AM,Gambari R,et al.Essential oils and isolated compounds from Lippia alba leaves and flowers: antimicrobial activity and osteoclast apoptosis[J].Int J Mol Med,2015,35(1):21121-21127
[8]BuenoSilva B,Koo H,Falsetta ML,et al.Effect of neovestitolvestitol containing Brazilian red propolis on accumulation of biofilm in vitro and development of dental caries in vivo[J].Biofouling,2013,29(10):1233-1242
[9]Chau NP,Pandit S,Jung JE,et al.Evaluation of Streptococcus mutans adhesion to fluoride varnishes and subsequent change in biofilm accumulation and acidogenicity[J].J Dent,2014,42(6):726-734
[10]Xu JS,Li Y,Cao X,et al.The effect of eugenol on the cariogenic properties of Streptococcus mutans and dental caries development in rats[J].Exp Ther Med,2013,5(6):1667-1670
[11]Daraghmeh NH,Chowdhry BZ,Leharne SA,et al.Chitin[J].Profiles Drug Subst Excip Relat Methodol,2011,36:35-102
[12]Aam BB,Heggset EB,Norberg AL,et al.Production ofchitooligosaccharides and their potential applications in medicine[J].Mar Drugs,2010,8(5):1482-1517
[13]Younes I,Sellimi S,Rinaudo M,et al.Influence of acetylation degree and molecular weight of homogeneous chitosans on antibacterial and antifungal activities[J].Int J Food Microbiol,2014,185:57-63
[14]Wu S.Preparation of chitooligosaccharides from Clanis bilineata larvae skin and their antibacterial activity[J].Int J Biol Macromol,2012,51(5):1147-1150
[15]Rahman MH,Shovan LR,Hjeljord LG,et al.Inhibition of fungal plant pathogens by synergistic action of chitooligosaccharides and commercially available fungicides[J].PLoS One,2014,9(4):e93192-
[16]Nguyen PT,Falsetta ML,Hwang G,et al.αMangostin disrupts the development of Streptococcus mutans biofilms and facilitates its mechanical removal[J].PLoS One,2014,9(10):e111312-
[17]周智,陈慧珍,胡德瑜.壳寡聚糖对变形链球菌影响的电镜观察[J].现代预防医学,2007,34(10):1823-1825

Inhibition of chitooligosaccharide on the cariogenicity of streptococcus mutans

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