牵张应变诱导人牙周膜细胞凋亡信号通路初探

Abstract

Abstract: Objective To explore the apoptosis mechanism of human periodontal ligament cells (HPDLCs) induced by Caspase under stretching force. Methods HPDLCs were cultured in vitro, and cells were stretched by dynamic mechanical strain 1%, 10% and 20% for 6 h, 12 h, 24 h and 48 h. The Caspase3 activity was measured after stretching in PDL cells that were treated with a specific inhibitor of either Caspase8 or Caspase9 prior to the application of stretching force. Results The activity of Caspase3 increased substantially in a time and force dependent manner after the application of a cyclic stretching force. It reached a peak at 24 h. The Caspase9 inhibitor abolished the stretching forceinduced activation of Caspase3, whereas the Caspase8 inhibitor demonstrated no effect. Conclusion Cyclic stretching force can induce apoptosis in HPDLCs through the activation of Caspase3 via the Caspase9 signaling cascade.

Key words: stretching force, apoptosis, Caspase, HPDLCs

CLC Number:

R781.4

References

[1]Zhong W,Xu C,Zhang F,et al.Cylic stretching forceinduced early apoptosis in human periodontal ligament cells[J].Oral Dis,2008,14(3):270-276
[2]Suo GJ,Qin J,Zhong ZX.Suppressor of cytokine signaling 1 inhibits apoptosis of islet grafts through caspase 3 and apoptosisinducing factor pathways in rats[J].Transplant Proc,2010,42(7):2658-2661
[3]Goga Y,Chiba M,Shimizu Y,et al.Compressive force induces osteoblast apoptosis via caspase8[J].J Dent Res,2006,85(3):240-244
[4]Creaqh EM.Caspase crosstalk: integration of apoptotic and innate immune signalling pathways[J].Trends Immunol,2014,35(12):631-640
[5]Snigdha S,Smith ED,Prieto GA,et al.Caspase3 activation as a bifurcation point between plasticity and cell death[J].Neurosci Bull,2012,28(1):14-24
[6]Nakamura K,BossyWetzel E,Burns K,et al.Changes in endoplas micreticulum luminal environment affect cells ensitivity to apoptosis[J].J Cell Biol,2000,150(4):731-740
[7]Mak BC,Wang Q,Laschinger C,et al.Nover function of PERK as a mediator of forceinduced apoptosis[J].J Biol Chem,2008,283(34):23462-23471
[8]Jang BG,In S,Choi B,et al.Betaamyloid oligomers induce early loss of presynaptic proteins in primary neurons by caspasedependent and proteasomedependent mechanisms[J].Neuroreport,2014,25(16):1281-1288
[9]Momeni HR,Soleimani MM,Shariatzadeh MA,et al.Caspasemediated apoptosis in sensory neurons of cultured dorsal root Ganglia in adult mouse[J].Cell J,2013,15(3):212-217
[10]赵彦超，顾耘.细胞凋亡通路研究进展[J].现代医学,2013,41(4):285-288
[11]Cai YM,Yu J,Gallois P.Endoplasmic reticulum stressinduced PCD and caspaselike activities involved[J].Front Plant Sci,2014,5(42):1-5
[12]Murray J,Renslo AR.Modulating caspase activity: beyond the active site[J].Curr Opin Struct Biol,2013,23(6):812-819
[13]Wen Q,Zhang X,Cai J,et al.A novel strategy for realtime and in situ detection of cytochrome c and caspase9 in Hela cells during apoptosis[J].Analyst,2014,139(10):2499-2506
[14]Rao RV,Hermel E,CastroObregon S,et al.Coupling Endoplas micreticulum stress to the cell death program:mechanism of Caspase activation[J].Biol Chem,2001,276(36):33869-33874
[15]Rao RV,CastroObregon S,Frankowski H,et al.Coupling endoplasmic reticulum stress to the cell death programAn Apaf1independent intrinsic pathway[J].. J Biol Chem,2002,277(24):21836-21842
[16]Wu Y,Wang D,Wang X,et al.Caspase 3 is activated through caspase 8 instead of caspase9 during H2O2induced apotosis in HeLa cells[J].Cell Physiol Biochem,2011,27(5):539-546

Study on stretching forceinduced apoptosis mechanism of human periodontal ligament cells

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