›› 2016, Vol. 36 ›› Issue (7): 603-606.

• Basic Research • Previous Articles     Next Articles

Effects of quercetin on the expression of IL-6 by LPSstimulated human periodontal ligament cells

  

  • Received:2015-05-28 Revised:2015-08-11 Online:2016-07-28 Published:2016-07-19

Abstract: Objective To observe the effects of quercetin on the expression of IL6 (interleukin6) by LPS (lipopolysaccharide) induced human periodontal ligament cells (hPDLCs), and to explore the antiinflammatory action of quercetin (Que). Methods hPDLCs were cultured in enzymic digestion tissue blocks and then identified. The inhibitory effects of quercetin with different concentrations on the expression of IL6 by LPSstimulated hPLDCs at different time points were assayed by ELISA (enzymelinked immunosorbent assay). Results IL6 secreted by hPLDCs peaked after the stimulation of LPS for 24 h. Compared with the LPS group, however, IL6 expressed by cells treated with quercetin before the stimulation of LPS significantly decreased with a statistical significance. No significant difference was found in different concentrations of quercetin groups. Conclusion Quercetin can inhibit the LPSinduced expression of IL6 in hPDLCs, suggesting that it may regulate the synthesis and secretion of IL6 in some way, thus alleviating inflammatory reaction in the early stage of inflammation.

Key words: lipopolysaccharide, enzymelinked immunosorbent assay, human periodontal ligament cells, quercetin, interleukin6

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