›› 2017, Vol. 37 ›› Issue (1): 25-28.

• Basic Research • Previous Articles     Next Articles

Inhibitory effect of tea polyphenols on H2O2 induced human periodontal ligament cell injury

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  • Received:2016-01-21 Revised:2016-04-08 Online:2017-01-28 Published:2017-01-17

Abstract: Abstract: Objective To study the protective effect of tea polyphenols (TP) on H2O2 induced human periodontal ligament cell injury and its mechanism. Methods Human periodontal ligament cell injury model was established using H2O2. Cells were randomly divided into three groups: control group, H2O2 group, and H2O2+ TP group. Cell viability was detected using MTT method and cell apoptosis was detected by flow cytometry. The MDA content and SOD activity were measured by the MDA and SOD detection kit. Protein expression levels of caspase-3, caspase-12, Bcl-2 and Bax were examined by western blot. The expression of IL-1β and IL-6 was detected by ELISA method. Results The cell viability was decreased with H2O2 treatment in a dose dependent manner. TP reversed the effect of H2O2 in human periodontal ligament cells, which included decrease of cell viability, increase of cell apoptosis, elevation of MDA, reduction of SOD activity, increase of expression levels of caspase-3, caspase-12, and Bax, decrease of Bcl-2 expression, and elevation of IL-1β and IL-6 expression. Conclusion The TP treatment protects H2O2 induced human periodontal ligament cell injury via inhibiting oxidative stress, mitochondrial apoptotic pathways and inflammation.

Key words: salidroside, periodontal ligament cells

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