三维打印超顺磁性氧化铁纳米组织工程支架

Abstract

Abstract: Objective To fabricate scaffolds containing superparamagnetic iron oxide nanoparticles（SPIONs） by 3D printing technology in order to make scaffolds with better biocompatibility for tissue engineering. Methods Gelatin-alginate hydrogel scaffolds adding SPIONs and not adding SPIONs were both fabricated by 3D printing technology. Stereomicroscope, transmission electron microscope（TEM）,vibrating sample magnetometer（VSM）and flow cytometry（FCM）were used to characterize the surface topography, internal structure, magnetic properties and biological properties. Results Stereomicroscope showed that scaffolds with regular structure and uniform pore could be successfully fabricated by 3D printing technology, and TEM showed that SPIONs were distributed evenly in the hydrogel matrix .The VSM results showed that scaffolds containing SPIONs exhibited superparamagnetism. The FCM results showed that compared with the control scaffolds, the cell proliferation index in the scaffolds in experimental group elevated, and the magnetic scaffolds could promote cell proliferation, which indicated that the magnetic ones had better biocompatibility. Conclusion Tissue engineering scaffolds can be successfully fabricated by 3D printing technology and scaffolds with SPIONs show better compatibility compared with those without SPIONs.

Key words: 3D printing, superparamagnetic iron oxide nanoparticles, tissue engineering, scaffolds

CLC Number:

R783.1

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Three dimensional printing SPIONs hydrogel scaffolds for tissue engineering

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