Abstract: Abstract: With the laser in oral medicine having increasingly wider range of application, attention to various types of laser in endodontic treatment has been higher. Root canal therapy is the first choice of treatment method of pulpitis and periapical disease, while the root canal cleaning is the key link to the success of root canal therapy. Er,Cr:YSGG laser, due to its disinfection, removal of smear layer on the root canal wall, and cleaning of root canal dentin debris and other infectious substances , becomes one of the most advantageous and promising lasers in the root canal cleaning-up. This article then reviewed the application of Er,Cr:YSGG laser in the root canal cleaning-up of root canal therapy, expecting to provide some help for the clinical application of this laser.