Abstract: Objective　To investigate the effect of Hydroxycamptothecin on the proliferation，apoptosis，migration and distribution of the cellcycle in FaDu after exposed to HCPT. Methods　Human pharyngeal squamous carcinoma cell line FaDu was cultivated. The growth inhibition was evaluated by MTT assay and the migration was measured by Transwell migration assay. The apoptosis and the cell cycle distribution were detected by flow cytometry after the treatment with HCPT. Results　MTT and Transwell experiments showed that certain concentration of HCPT could effectively inhibit the growth and migration of cell line FaDu. According to the results of flow cytometry，the cell cycle was arrested in S phase and apoptosis was obviously induced after treated with HCPT(80ng/ml).Conclusions　HCPT can inhibit the growth and migration of cell line FaDu and induce its apoptosis. HCPT induced apoptosis of the cell cycle in FaDu is probably connected with its effect on the cell cycle of FaDu.

Key words: Hydroxycamptothecin, human pharyngeal squamous cell carcinoma cell line FaDu, Apoptosis