›› 2017, Vol. 37 ›› Issue (4): 293-297.

• Basic Research • Previous Articles     Next Articles

Influence of calcium silicate compound with calcium magnesium phosphate cement on human osteoblast initial adhesion

  

  • Received:2016-11-20 Revised:2016-12-23 Online:2017-04-28 Published:2017-04-11
  • Contact: Qing-Yi SHEN E-mail:est_s@sina.com

Abstract: Objective To compare the initial adhesive ability of osteoblast which adheres to calcium magnesium phosphate cement (CMPC) and calcium silicate compound with calcium magnesium phosphate cement (CMPSiC). Methods The ions concentrations of the extract from CMPC and CMPSiC were detected. The initial attachment effects of MG63 on CMPC and CMPSiC were performed by MTT and cell morphometric evaluations. Results Concentrations of calcium, magnesium and phosphorus ion of the CMPC and CMPSiC extract significantly increased after immersion for 24 hours(P<0.05). And concentration of silicon ion significantly increased in CMPSiC extract(P<0.05). The result of MTT experiment indicated that the number of osteoblasts increased over time, and the number adhering on CMPSiC surface was significantly more than that on CMPC surface (P<0.05). After 6 h inoculation, cells adhering on CMPC surface were shrinked, and those on CMPSiC surface were spreading. Cell morphological parameters such as cell surface area, perimeter and Fred diameter of CMPSiC group were significantly larger than those of CMPC group(P<0.05). Conclusion Compared with CMPC, CMPSiC is more conducive to initial adhesion and spread of osteoblast.

Key words: adhesion behavior, calcium silicate, calcium phosphate cement, magnesium phosphate cement

CLC Number: