BMSCs和FG作为种子细胞和支架材料修复大鼠牙槽骨缺损的效果

Abstract

Abstract: Objective To investigate the effects of bone marrow mesenchymal stem cells (BMSCs) and fibrin glue (FG) as seed cells and scaffold materials in repairing alveolar bone defect in rats. Methods 30 SD rats were selected to establish the alveolar bone defect model， randomly divided into observation group， negative control group and blank control group， with 10 rats in each group. In the observation group， BMSCs and FG were implanted to repair the alveolar bone defect of the rats; while the negative control group was implanted with FG， and the blank control group was not implanted. HE staining and MicroCT scanning were used to observe the osteogenesis effect of each group. Results After 6 weeks in the observation group， the bone healing was good， and the healing surface was smooth; while in the negative control group and blank control group， the bone defect still existed， and there was no obvious bone formation. The percentage of new bone area in the observation group was (76.23±7.11)%， significantly higher than that of the negative control group and blank control group， and the difference was statistically significant (P<0.05). MicroCT scanning showed that in the observation group， new bone formed in the area where bone defect existed， bone defect basically healed， and there was no obvious boundaries around. The negative control group and blank control group had no obvious osteogenesis， and the bone defect was still obvious. Conclusion BMSCs and FG as seed cells and scaffold materials for repairing alveolar bone defects have good effects， which can improve the new bone quantity， and are the ideal materials for the repair of alveolar bone defects.

Key words: bone marrow mesenchymal stem cells, fibrin glue, alveolar bone defect, rat

CLC Number:

R782.4

References

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Effects of BMSCs and FG as seed cells and scaffold materials on repairing alveolar bone defect in rats

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