DLK1对人牙髓干细胞增殖和成牙本质分化的影响

Abstract

Abstract: Objective　To investigate the functions of DLK1 in the proliferation and differentiation of human dental pulp stem cells (hDPSCs). Methods Immunohistochemical analysis was used to determine the expression of DLK1 in the mouse first maxillary molar. Recombinant lentivirus was constructed to overexpress DLK1 stably in hDPSCs.The cell viability and proliferation of hDPSCs were examined by CCK8 and EdU incorporation assay, respectively. The odontoblastic differentiation of hDPSCs was determined by detection of ALPase activity assay, ALP and alizarin red staining and the expression of mineralization-related genes including ALP, DSPP and DMP1. Result DLK1 was highly expressed in the odontoblasts and dental pulp cells on the first maxillary molar. The expression level of ALP,DSPP,DMP-1 and DLK1 increased significantly after the odontoblastic induction of hDPSCs. Compared with the control group, the expression level of ALP increased by 1.6 times, DSPP increased by 1.16 times, DMP1 increased by 1.42 times and DLK1 increased by 1.54 times. DLK1 over expression increased the proliferation ability of hDPSCs and inhibited odontoblastic differentiation of hDPSCs. Compared with the control group, hDPSCs increased by about 1.7 times with the over expression of DLK1. Conclusions The proliferation of hDPSCs is promoted after DLK1 over expression. DLK1 inhibits the odontoblastic differentiation of hDPSCs.

Key words: hDPSCs, DLK1, proliferation, odonoblastic differentiation

CLC Number:

　R781

References

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Effect of delta-like 1 homologue on the proliferation and dontoblastic differentiation in human dental pulp stem cells

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