Abstract: 【Abstract】　Objective: This study aims to examine whether human dental pulp stem cell (DPSC) can be induced to differentiate into smooth muscle cell (SMC) in vitro. Method: The various concentration of condition medium (CM) from SMC and the growth factor of transforming growth factor beta 1 (TGF-β1) were evaluated to ascertain which were the most potent inducers of differentiation. DPSCs were induced to differentiate into SMC by growth environment containing SMCs-CM with the addition of TGF-??1 for 14 days. Results: The morphology of DPSC was demonstrated to change from the spindle shape to the "hill and valley" morphology of SMCs over time. The gene and protein expression of SMCs-specific markers (α-SMA, desmin, myosin, and calponin) increased over time. Conclusions: These data demonstrated that DPSC has the potential to differentiate into the SMC, which represents a step forward in providing a promising alternative source of cells that are obtained by least invasive procedure without ethical issues, for future research in SMC tissue engineering.

Key words: 【key words】Dental Pulp Stem Cells, Smooth Muscle Cell, Tissue Engineering