Abstract: Abstract：Objective To investigate the effect of miR-145 on the osteogenesis differentiation of rBMMSC. Methods Rat shin bone marrow mesenchymal stem cells collected from 3-week male rats were divided into two groups, pLv3-Negative control group and pLv3-(anti)miR-145 group, transfected with lentivirus. The effects of miR-145 on cell proliferation, apoptosis, growth cycle and osteogenesis differentiation of rBMMSC were observed. Results There was no difference in cell proliferation, growth cycle and apoptosis between pLv3-Negative control group and pLv3-(anti)miR-145 group. The expressions of Sema3A, OPG, Runx2 and Osterix/SP7 in pLv3-(anti)miR-145 group were significantly higher than pLv3-Negative control group. The area of Alizarin red staining in pLv3-(anti)miR-145 group was also bigger than pLv3-Negative control group. Conclusion Inhibition of miR-145 expression can enhance osteogenesis differentiation of rBMMSC，but it has no obvious effect on cell proliferation, growth cycle and apoptosis of rBMMSC.

Key words: Key words: miR-145, rBMMSC, Osteogenesis differentiation