Abstract: Objective To explore the physicochemical and biological properties of different colloidal materials combined with antler powder and nanohydroxyapatite (nHA) scaffolds. Methods Antler powder and nano hydroxyapatite meal were mixed with 15% PVA, 5% silk fibroin (SF), 15% PVA and 5% silk fibroin (4∶1) respectively to prepare antler powder/PVA scaffolds, antler powder/SF scaffolds,antler powder/PVA/SF scaffolds, nano hydroxyapatite (nHA)/PVA scaffolds, nanohydroxyapatite (nHA)/SF scaffolds, nanohydroxyapatite (nHA)/PVA/SF scaffolds. The antidissolution performance, anticompression force, shear resistance and scaffold cytotoxicity were measured. Results The dissolution deformation of antler powder/SF scaffolds and nHA/SF scaffolds were grade Ⅲ, and the other four groups were grade Ⅱ. The shear resistance and anticompression force of the antler powder/SF scaffolds and nHA/SF scaffolds were poor. The antishear properties of the antler powder/PVA scaffolds and antler powder/PVA/SF scaffolds were similar to those of the nHA/PVA scaffolds and nHA/PVA/SF scaffolds. The anticompression properties of antler powder/PVA scaffolds and antler powder/PVA/SF scaffolds were better than nHA/PVA scaffolds and nHA/PVA/SF scaffolds, respectively. Conclusions Antler powder composite scaffolds have good mechanical properties and cell compatibility, and can be used as a new direction for heterogeneous bone scaffold materials. Pure silk fibroin, as a composite scaffold with poor solubility, poor mechanical properties, cannot meet the requirements of bone tissue engineering scaffolds.

Key words: antler powder, nanohydroxyapatite, scaffold material, polyvinyl alcohol, silk fibroin solution