辣椒素对牙龈卟啉单胞菌生长及实验性牙周炎大鼠炎性因子分泌的影响

Abstract

Abstract: Objective To investigate the effect of Cap on the pg growth and inflammatory cytokine secretion in experimental rat periodontitis. Methods Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Cap against pg (ATCC33277) were determined by microdilution technique. The effect of Cap on the growth rate of pg was assessed by timekill curve. By placing the sterile 28gauge wire ligature to the cervical portion of the right first molar, the experimental animal periodontitis was set up. The levels of TNFα, IL1β, IL4, IL6, IL10, IL12 in the periodontal tissues were analyzed by ELISA. Results Statistical analysis was performed using SPSS19.0 software. It was found that Cap showed the inhibitory effects on pg growth with MIC at 16mg/L and 64 mg/L, respectively. In low concentration, Cap inhibited pg growth, but in high concentration, Cap could directly kill bacteria. The inhibitory or killing effect of Cap was also in timedependent manners. In experimental periodontitis, except IL4, TNFα, IL1β, IL6, IL12 were significantly inhibited by Cap, but the expression of IL10 increased. Conclusion Cap can inhibit the growth of pg. By selectively changing the expression of TNFα, IL1β, IL6, IL10, IL12, Cap is able to relieve the immuneinflammatory reaction in the periodontitis.

Key words: porphyromonas gingivalis, capsaicin, growth, experimental periodontitis

CLC Number:

R781.42

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Effects of capsaicin on porphyromonas gingivalis growth and inflammatory cytokine secretion in experimental rat periodontitis

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