白介素-17对破骨前体细胞RAW264.7增殖分化的影响

Abstract

Abstract: To explore the effects of IL-17A to promote proliferation and differentiation of preosteoclasts RAW264.7 cells. Methods: RAW264.7 cells were cultured by RANKL and M-CSF at 24h after treated with IL-17A. CCK-8 kits and RT-PCR were used to evaluate the effects of proliferation and differentiation. Results: IL-17A could promote proliferation of RAW264.7 cells, the proliferation effects of IL-17A had a dose-effect relationship and was in a time-dependent manner; At the concentration of 50ng/ml of IL-17A, the activity of genes including CathK，RANK and NAFTC1 mRNA increased. Conclusion: It is demonstrated that IL-17A mainly promotes RAW264.7 cells proliferation, and promotes RAW264.7 cells differentiation.

Key words: IL-17A, RAW264.7, proliferation, differentiation

CLC Number:

R349.5

References

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Influence of IL-17 on RAW264.7 cells proliferation and differentiation

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