miR-335对牙囊干细胞成骨分化能力的影响

Abstract

Abstract: Objective To study the osteogenic differentiation potential of dental follicle stem cell (DFSCs) in virto and the effects of miR335 on the osteogenic differentiation potential of DFSCs. Methods DFSCs were purified through in vitro culture combined with bidirectional differential method. After osteogenesis induction, the osteogenic differentiation potential was determined by alizarin red staining. miR335 expression was analyzed by qRTPCR. Western blot and PCR were used to evaluate the osteogenic differentiation potential of DFSCs after transient transinfection of miR335 mimics or inhibitor. Results DFSCs cultured by bidirectional differential method had osteogenesis ability. miR335 expression in DFSCs was lower than that in osteogenic induced DFSCs. The osteogenic differentiation potential of DFSCs was reduced by miR335 mimics, while promoted by miR335 inhibitor. Conclusion miR335 in virto may have a negative effect on the ostegenic differentiation of DFSCs.

Key words: miR-335, dental follicle stem cells, osteogenic differentiation

CLC Number:

R329.29

References

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The effects of miR-335 on the osteogenic differentiation potential of the DFSCs

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