具核梭杆菌感染KB细胞影响细胞增殖和炎症因子分泌的研究

Abstract

Abstract: Objective Fusobacterium nucleatum (F. nucleatum), Gramnegative bacteria, is one of the important pathogens of periodontal disease. The studies have found that periodontal pathogen infection and oral cancers development are closely related. Therefore, this study aims to analyze the effects of bacterial infection on cell proliferation and cytokine secretion, providing new evidence for the relationship between F. nucleatum infection and oral cancer development. Methods KB cells infected with F. nucleatum model was established. The morphology of bacteria and cell was observed by transmission electron microscopy. Cell cycle was analyzed by PI staining flow cytometry, the levels of IL6 and IL8 cytokines in the supernatant were detected by ELISA. Results F. nucleatum invaded the cells at 4 h. The bacteria remained intact within the cells, and the tight connection was broken among cells. 24 h after infection, F. nucleatum could still be found within the cells. The bacteria capsule structure wrapped around and maintained integrity. Low concentration of F. nucleatum (MOI=10) infected KB cells promoted G1 phase process at 4 h(P<0.05), but had no significant changes in cell proliferation. High concentration of F. nucleatum (MOI=100) infected KB cells promoted cell proliferation through accelerating S phase cells at 4 h8 h. However, after 24 h infection, the G1 phase was inhibited and the S phase was significantly reduced resulting in cell proliferation blocked (P<0.05). The levels of IL6 and IL8 cytokines in the supernatant increased and the time and concentration were dependent on the KB cells with F. nucleatum. Conclusions Infection of oral cancer cells with F. nucleatum may aggravate the malignant course of cancer cells by affecting cell proliferation and cell cycle progression, exacerbating cell inflammatory responses.

Key words: Fusobacterium nucleatum, cell cycle, cytokines, tumor cell

CLC Number:

R781.4

References

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Effect of Fusobacterium nucleatum on cell proliferation and production of inflammatory factors in KB cells

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