Abstract: Abstract：Objective To establish a murine oral mucosal candidiasis model, observe oral mucosal infection and examine the corresponding systemic infectiondynamically, and to analyzethe changes of the infection. MethodsImmuno-suppressed mice were inoculated with C.albicansinfectionusing the oral swabs. Lesions in the murine tongue were observed using macroscopic observation. The viable cells of the C. albicans in the oral cavity were calculated and the histological study of the murine tongues was analyzed to determine C. albicans infections of the oral mucosal. Weight and fungal burdens in the kidney, liver and feces from the descending colon were examined to assess the severity of the systemic infections. The above indicators in the infected mice were examined and recorded for a week after inoculation. In the blank group immunosuppressed mice were swabbed with saline. Results①The number of viable C. albicans cells in the oral cavity was low on the 1st day after inoculation，then rapidly grew and tended to be stable between the range of 106-107CFU / ml from day 3 to day 7 after inoculation . Oral tongue lesions had a similar trend. There was no obvious pseudomembranous on day 1 and visible tongue dorsal pseudomembranous could be seen from day 3 to day 7 after inoculation .②Histopathological sections stained by PAS revealed that C. albicans had developed a mycelial form, penetrated into the mucosal epithelium and destroyed the epithelium within 3-5 days after inoculation. There were visible yeast cells on the mucosal epithelium on day 7 after inoculation.③The weight of the mice gradually decreased after inoculation. Candida cells that recovered from feces gradually increased and reached to the highest level on the 5th day afterinoculation, but in kidneys and livers , there were no viable Candida cells . The blank group was not infected with C. albicans . Conclusions：Inoculating C. albicans in the oral cavity of mice using oral swabs can establish a murine oral mucosal candidiasis model. The oral candidiasis of the infected mice has changes in a week. The appropriate time of animal experiments should have been chosen depending on the infection status

Key words: 【Key words】Candida albicans, Murine oral candidiasis, Mucosal infection