CCR6-CCL20轴在口腔扁平苔藓黏膜组织中的表达及意义

Abstract

Abstract: Abstract： Objective This study was undertaken to detect variation of CCR6-CCL20 expression in tissue from OLP patients and healthy controls. Methods Real-time quantitative PCR was used to detect the mRNA expression of CCR6 and CCL20 in oral mucosal tissue and immunohistochemistry was used for the detection and localization of CCR6 and CCL20. Results Tissue from OLP patients had markedly up-regulated levels of CCR6 and CCL20 mRNA compared to healthy tissue (P < 0.001). Moreover, immunohistochemical staining showed that in OLP patients, CCR6 and CCL20 expression was obviously higher than that in the control patients. Conclusion The CCR6CCL20 axis may play a role in the immunological mechanism of OLP. [Key words] oral lichen planus; CCR6; CCL20

Key words: oral lichen planus, CCR6, CCL20

CLC Number:

R781.5

References

[1]Olson MA, Rogers RS, Bruce AJ.Oral lichen planus[J].ClinDermatol, 2016, 34(4):495-504
[2]Firth FA, Friedlander LT, Parachuru VP, et al.Regulation of immune cells in oral lichen planus[J].Arch DermatolRes, 2015, 307(4):333-339
[3]Kurago ZB.Etiology and pathogenesis of oral lichen planus: an overview[J].Oral Surg Oral Med Oral Pathol Oral Radiol, 2016, 122(1):72-80
[4]Payeras M R, Cherubini K, Figueiredo M A, et al.Oral lichen planus: focus on etiopathogenesis[J].Arch Oral Biol, 2013, 58(9):1057-1069
[5] J.W. Griffith, C.L. Sokol, A.D. Luster. Chemokines and chemokine receptors: positioning cells for host defense and immunity[J]. Annu Rev Immunol, 2014(32): 659-702.
[6]Zlotnik A, YoshieO.The chemokine superfamily revisited[J].Immunity, 2012, 36(5):705-716
[7]Schutyser E, Struyf S, Van Damme J.The CC chemokine CCL20 and its receptor CCR6[J].Cytokine & growth factor reviews, 2003, 14(5):409-426
[8]Schmuth M, Neyer S, Rainer C, et al.Expression of the C-C chemokine MIP-3 alphaCCL20 in human epidermis with impaired permeability barrier function[J].Exp Dermatol, 2002, 11(2):135-142
[9]Lee AY, Eri R, Lyons AB, Grimm MC, Korner H.CC Chemokine Ligand 20 and Its Cognate Receptor CCR6 in Mucosal T Cell Immunology and Inflammatory Bowel Disease: Odd Couple or Axis of Evil?[J]. Front Immunol, 2013, 4:194.
[10]Kalinowska-Lyszczarz A, Szczucinski A, Pawlak M A, et al.Clinical study on CXCL13,CCL17,CCL20 and IL-17 as immune cell migration navigators in relapsing-remitting multiple sclerosis patients[J].J Neurol Sci, 2011, 300(1-2):81-85
[11] Lu R, Zeng X, Han Q, et al.Overexpression and selectively regulatory roles of IL-23/IL-17 axis in the lesions of oral lichen planus[J]. Mediators of inflammation, 2014, 2014: 701094.
[12]Meares GP, Ma X, Qin H, et al.Regulation of CCL20 expression in astrocytes by IL-6 and IL-17[J].Glia, 2012, 60(5):771-781
[13]Hirota K, Yoshitomi H, Hashimoto M, et al.Preferential recruitment of CCR6-expressing Th17 cells to inflamed joints via CCL20 in rheumatoid arthritis and its animal model[J].J Exp Med, 2007, 204(12):2803-2812
[14]Harper EG, Guo C, Rizzo H, et al.Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis[J].J Invest Dermatol, 2009, 129(9):2175-2183
[15]Di Cesare A, Di Meglio P, Nestle FO.The IL-23Th17 axis in the immunopathogenesis of psoriasis[J].J Invest Dermatol, 2009, 129(6):1339-1350
[16] Bullens DM, Truyen E, Coteur L, et al.IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulocytic influx? [J].Respir Res, 2006, 7:135.
[17]Parashar P.Oral lichen planus[J].Otolaryngol Clin North Am, 2011, 44(1):89-107
[18]Livak KJ, Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J].Methods, 2001, 25(4):402-408
[19]Lu R, Zhang J, Sun W, et al.Inflammation-related cytokines in oral lichen planus: an overview[J].J Oral Pathol Med, 2015, 44(（1):1-14
[20]Hu JY, Zhang J, Cui JL, et al.Increasing CCL5CCR5 on CD4+ T cells in peripheral blood of oral lichen planus[J].Cytokine, 2013, 62(1):141-145
[21]Komiya T, Sugiyama T, Takeda K, et al.Suppressive effects of a novel CC chemokine receptor 4 antagonist on Th2 cell trafficking in ligand- and antigen-induced mouse models[J].Eur J Pharmacol, 2013, 720(1-3):335-343
[22]Rostami A, Ciric B.Role of Th17 cells in the pathogenesis of CNS inflammatory demyelination[J].J Neurol Sci, 2013, 333(1-2):76-87
[23]Shaker O, Hassan AS.Possible role of interleukin-17 in the pathogenesis of lichen planus[J].Br J Dermatol, 2012, 166(6):1367-1368
[24]Monteiro B V, Pereira Jdos S, Nonaka C F, et al.Immunoexpression of Th17-related cytokines in oral lichen planus[J].Appl Immunohistochem Mol Morphol, 2015, 23(6):409-415
[25]Zhang W, Nie L, Wang Y, et al.CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues[J].PLoS One, 2013, 8(6):e66286-
[26] Caballero-Campo P, Buffone MG, Benencia F, et al.Arole for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis[J]. J Cell Physiol, 2014, 229(1):68-78.

CCR6 -CCL20 expression profile in peripheral blood and tissue of Oral Lichen Planus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments

[1]	. Meta-analysis and systematic evaluation of the efficacy of combined total glucosides of paeony capsules in the treatment of oral lichen planus [J]. , 2019, 39(6): 523-529.
[2]	. Efficacy observation of Er,Cr:YSGG laser with LLLT mode on treatment of erosive oral lichen planus [J]. , 2019, 39(2): 135-137.
[3]	. Expression of KLF-6 in the patients with oral lichen planus canceration [J]. , 2018, 38(2): 132-134.
[4]	. Study on the immunoregulation mechanism of Astragalus Polysaccharide in oral lichen planus [J]. , 2017, 37(7): 593-597.
[5]	. Impact of basic periodontal therapy on the peripheral blood IL-10 and TGF-β levels in oral lichen planus patients [J]. , 2017, 37(1): 53-56.
[6]	. Curative effects and security of tacrolimus on oral lichen planus：A systematic review [J]. , 2017, 37(1): 38-45.
[7]	. Observation of RANTES in various types of abnormal humour syndrome patients with oral lichen planus [J]. , 2016, 36(8): 686-688.
[8]	. Expression of IFN-γ, IL-10 and IL-17 in patients with oral lichen planus [J]. , 2016, 36(7): 612-614.
[9]	. Application of chronic disease management in common oral diseases [J]. , 2016, 36(3): 262-265.
[10]	. Expression of TL1A and IDO in the peripheral blood in the patients with oral lichen planus [J]. , 2015, 35(4): 278-281.
[11]	. Research progress of the relationship between oral lichen planus and periodontitis [J]. , 2015, 35(1): 71-75.
[12]	Hui PENG. [J]. , 2014, 34(11): 867-869.
[13]	. Clinical study of Huashi Xingyu Qingre decoction in treatment of oral lichen planus [J]. , 2014, 34(10): 730-733.
[14]	. Clinical evaluation of total glucosides of paeony combined with triamcinolone acetonide for oral lichen planus [J]. , 2014, 34(1): 56-58.