Abstract: Objective The aim of this study was to evaluate the effects of Asperosaponin D(ASD) on periodontium remodeling as well as the mechanism of effects on osteoclast. Methods In all, forty Wistar rats were selected and randomly divided to four groups (n=10). The control group (Group A) received no drug, the experimental Group B received a low concentration ASD (5 mg/kg) injection, the experimental Group C received a high concentration ASD (10 mg/kg) injection and group D received an appropriate concentration PGE2 (25μg/kg). The 5 groups of rats were sacrificed in batch on the 3st, 7th, 14th, 21st and 28th day with cardiac perfusion. Osteoclastic activity in the first molar periodontal tissue was evaluated by TRAP stainings and immunohistochemical staining. All statistical analysis was performed using SPSS software version 13.0. Results From 3rd to 21st, TRAP staining and immunohistochemical analysis of periodontium by the expression of ODF showed that the osteoclast numbers of every groups increased. From 7th days to 28th days, compared with group A, the number of osteoclast in the experimental group(Group B, C, D) increased. the number of osteoclast in group B was less than in group C and group D, and there were similar with group C and group D. Conclusions Local injection of both low and high concentrations of ASD and PGE2 may promote OTM by enhancing osteoclastic activity. The effect of local injection of high concentrations of ASD and PGE2 is similar, however The effect of high concentrations of ASD solution is more obvious than the effect of concentrations of ASD solition.

Key words: Asperosaponin D, orthodontic tooth movement, osteoclast differentiation, periodontium remodeling