Abstract: Abstract: Objective To detect human papilloma virus (HPV) genotypes in oral leukoplakia (OLK) and oral squamous cell carcinoma (OSCC) and its clinical significance. Methods Polymerase chain reaction（PCR）and diversion hybridization technology were utilized to detect 37 kinds of HPV genotypes in oral mucosa exfoliated cells from 30 cases of normal mucosa,103 cases of OLK and 30 cases of OSCC, respectively. Clinicopathological characteristics of all subjects were also analyzed. Results In normal oral mucosa, HPV detection rate was 3.33% (1/30). In OLK, HPV detection rate was 4.85% (5/103). In OSCC, HPV detection rate was 3.33% (1/30). There was no statistically significant difference among the groups (chi-square =0.22, P=0.90 > 0.05). 4 OLK cases were detected with 1 kind of HPV, and 1 case was detected with 3 kinds of HPV. Conclusions Non-invasive sampling with PCR and diversion hybridization technology can be applied to clinically detect HPV genotypes in oral mucosa. The detection rates of HPV in OLK and OSCC are low, and HPV may not be the main pathogenesis factor.

Key words: Keywords: oral leukoplakia, oral squamous cell carcinomas, human papilloma virus, genotypes