脂肪干细胞复合成骨性干细胞膜片用于修复兔颅骨缺损的实验研究

Abstract

Abstract: Objective　Cell sheet technology has been proved successful for multiple tissue regeneration. Our previous study demonstrated that adipose-derived stem cells (ADSCs) can significantly promote the ectopic osteogenic potential of osteoblastic bone marrow mesenchymal stem cell (BMSCs) sheet. Therefore, the purpose of this study was to verify whether ADSCs can promote bony healing potential of BMSCs sheet in calvarial defects in rabbits. Methods ADSCs and BMSCs were obtained from the same donor's inguinal fat and iliac cancellous bone, respectively. BMSCs were cultivated by continuous osteoinduction method for two weeks, and the characteristics of BMSCs sheet were analyzed. Then the combination of ADSCs and BMSCs sheet was transplanted to calvarial defects in rabbits. BMSCs sheet and calvarial defects without any treatment were considered as control groups. Eight weeks after the transplantation, the bony restoration and regeneration were assessed by microcomputed tomography (Micro-CT) scanning and histological analysis. Results BMSCs sheet consisted of multilayer sheets containing a large number of mineralized calcium nodules. After eight weeks of transplantation, compared to control groups, BMSCs sheet with ADSCs increased better bone regeneration as evidenced by the greater density of bone, larger number of newly formed mineralized tissue and greater bone volume / total volume (BV / TV) ratio within the composite constructs. Conclusions The combination of ADSCs and BMSCs sheet can significantly promote bone regeneration in calvarial defects in rabbits, which provides a new way to improve bone healing.

Key words: Adipose-derived stem cells, Cell sheet, Calvarial defects, Bone regeneration

CLC Number:

　R329.28

References

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An experimental study of the application of adipose-derived stem cells compositing osteoblastic mesenchymal stem cell sheets to improveing bone formation in calvarial defects in rabbits

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