Abstract: Objective: To study the DI value and ploidy distribution of oral squamous cell carcinoma (OSCC) by ICM-DNA quantitative analysis system, and to evaluate its relationship with clinicopathological factors of OSCC. Methods: 79 paraffin specimens of OSCC were collected as experimental group, 25 cases of normal oral mucosa tissue and 30 cases of oral mucosa epithelial dysplasia as control group.ICM-DNA quantitative analysis was carried out after Feulgen staining. Results: 79 cases of OSCC in the aneuploidy occurrence rate was 55.7% (44/79), were significantly higher than the control group of 0% normal tissues (0/25) and oral mucosa epithelial dysplasia (14/30) 46.7%, P =0.000; Compared with mild , moderate and severe three groups of oral mucosa epithelial dysplasia , the rate of aneuploidy occurrence was 0 % ( 0 / 2 ) , 14.3 % ( 1 / 7 ) , 61.9 % ( 13 / 21 ) , P = 0.036 , respectively ;high and moderate-poor differentiation between the three groups, aneuploidy occurrence rate of high differentiation group at the rate of 42.5% (20/47) lower than the moderate-poor differentiation group was 75% (24/32), P =0.004; lymph node metastasis group have 78.6% aneuploidy occurrence rate (22/28), higher than that without lymph node metastasis 43.1% (22/51),P =0.002.The incidence of aneuploidy was not associated with age (P=0.193), sex (P=0.232) ,smoking (P=0.151) and the location of the disease (P=0.915). Conclusion: DNA aneuploidy rate of OSCC was higher than that of normal tissue and epithelial dysplasia of oral mucosa, and may be associated with the degree of differentiation and lymph node metastasis .ICM-DNA quantitative analysis provides a valuable clinical indicator for the diagnosis of OSCC and the judgment of malignancy ,which is expected to be used for adjuvant clinical prediction and the diagnosis of OSCC

Key words: oral squamous cell carcinoma, ICM, DNA quantitative analysis, aneuploidy