iRoot BP Plus对骨髓间充质干细胞成牙/成骨分化及自噬的影响

Abstract

Abstract: Objective: To investigate the effect of iRoot BP Plus on the odonto/osteogenic differentiation and autophagy of bone marrow mesenchymal stem cells. Methods: BMMSCs were harvested by the whole marrow method. iRoot BP Plus conditioned medium was made by material evaluates and the optimal concentration of iRoot BP Plus conditioned medium was determined by western blot. ALP staining, qRT-PCR and western blot were used to investigate the odonto/osteogenic capacity of BMMSCs. The autophagy related makers wasere detected by western blot. Results: 0.2 mg/ml was the optimal concentration of iRoot BP Plus conditioned medium. When treated with 0.2 mg/ml iRoot BP Plus conditioned medium, the expression of odonto/osteogenic markers in BMMSCs was significantly higher than that in control group, and the ratio of LC3Ⅱ/LC3Ⅰupregulated while the protein level of p62 decreased. Conclusion: iRoot BP Plus can potentially promote the odonto/osteogenic capacity of BMMSCs through activating autophagy.

Key words: Root BP Plus, bone marrow mesenchymal stem cells, the odonto/osteogenic differentiation, autophagy

References 34

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Effect of iRoot BP Plus on the odonto/osteogenic differentiation and autophagy of bone marrow mesenchymal stem cells

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