DKK-1在大鼠牙囊细胞体外的表达作用研究

Abstract

Abstract: Abstract： Objective To study the regulating protein of Wnt signaling pathway, DKK-1, inhibitor can be expressed in the dental follicle cells. To explore the specific expression mechanism of DKK-1 during osteogenic differentiation of dental follicle cells and the relationship between the formation of mature osteoblasts and eruption of the teeth. Methods The dental follicle cells were primarily cultured by digestion method and purified. The cells were divided into 2 groups: the control group was cultured in α-MEM medium, and the experimental group was cultured in α-MEM medium supplemented with osteogenesis induction. With increased of time after intervention two groups of cells were identified using immunocytochemistry. Western-blotting was used to evaluate the lever of cellular DKK-1、β-catenin and Runx2 protein. Results Compared with control group, the factors of DKK-1 expression of experimental group was significantly lower, the Western-blot results showed that the expression of DKK-1 were significantly lower than that of the control group, but the osteogenic factor Runx2 protein、expression was enhanced in the experimental group over time. Conclusions The expression of inhibitor DKK-1 a key regulator of Wnt pathway, is affected by osteogenesis induced differentiation in the dental follicle cells and reflect its inhibitory characteristics. To suggests the molecular basis of the formation mechanism for the development of Maxillary and mandible and eruption of the teeth.

Key words: Key words： DKK-1, the dental follicle cells, Wnt/β-catenin, Osteoblast, osteoclast

CLC Number:

R341.8

Lu YAN. In vitro study of the effects of DKK-1 on the dental follicle cells [J]. , 2019, 39(10): 902-906.

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In vitro study of the effects of DKK-1 on the dental follicle cells

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