›› 2019, Vol. 39 ›› Issue (3): 199-202.

• Basic Research • Previous Articles     Next Articles

Antibacterial properties research on HAPw/nmZnO-nmCaO composite bone restorative material in vitro

  

  • Received:2018-09-19 Revised:2018-10-19 Online:2019-03-28 Published:2019-03-22
  • Contact: Jing LI E-mail:515384248@qq.com

Abstract: Researched on antibacterial properties of HAPw/nmZnO-nmCaO against Staphylococcus aureus and Pseudomonas aeruginosa in vitro. Material and methods:Prepared Staphylococcus aureus and Pseudomonas aeruginosa suspension,OD595=0.1.HAPw、Bio-oss、HAPw/nmZnO-nmCaO material suspension prepared with 1mg/ml of sterile pure water.Group A: 5 ml medium + 1 ml HAPw/nmZnO-nmCaO + 200ul bacterial suspension; Group B: 5 ml medium + 1 ml HAPw + 200ul bacterial suspension; Group C: 5 ml medium + 1 ml Bio-oss + 200ul bacterial suspension; D control group: 5 ml medium + 1 ml sterilized pure water + 200 ul bacterial suspension.Each group was incubate 20h in thermostat with temperature of 37℃,then the samples were diluted with a concentration gradient. The diluted mycelium was coated on the agar plate with 100ul and incubate 20h in thermostat with temperature of 37℃,the plate colonies were counted..Results:Staphylococcus aureus and Pseudomonas aeruginosa HAPw/nmZnO-nmCaO group had fewer colonies than HAPw, Bio-ose, control group(P<0.001),there was no siginificant difference between HAPw, Bio-oss, and control group(P>0.05).Conclusions:HAPw/nmZnO-nmCaO inhibited the growth of Staphylococcus aureus and Pseudomonas aeruginosa. The inhibition of Staphylococcus aureus was stronger than that of Pseudomonas aeruginosa.

Key words: HAPw/nmZnO-nmCaO, inoganic antiseptic, antibacterial property

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