煅烧牙粉影响人牙周膜干细胞体外矿化的自噬机制研究

Abstract

Abstract: Object: To evaluate the effects of calcined tooth powder on mineralization of human periodontal ligament stem cells (hPDLSCs) in vitro and related mechanism. Method: The hPDLSCs were isolated and cultured with or without calcined tooth powder severally．The apoptosis of hPDLSCs was detected by flow cytometry. Western blot, immunofluorescence staining, alizarin red staining and alkaline phosphatase staining were performed to verify the effect of calcined tooth powder on autophagy and mineralization of hPDLSCs in vitro. Result: There are no significant differences of apoptosis between two groups of hPDLSCs. The protein level of Beclin1, ATG5 and the ratio of LC3II/LC3I of hPDLSCs cultured in calcined tooth powder conditioned medium were significantly higher than the control group hPDLSCs, while the expression of P62 was significantly lower than the control group. The calcium mineralization was significantly higher after osteogenic induction in the group of hPDLSCs calcined tooth powder treated than control group. Alkaline phosphatase expression levels were inhibited by inhibition of autophagy in the process of mineralization in vitro. Conclusions：The calcined tooth powder could up-regulate mineralization of hPDLSCs via inducing autophagy in vitro．

Key words: ［Key words］calcined tooth powder, human periodontal ligament stem cells, autophagy, mineralization

CLC Number:

R780.2

References 29

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A study on the effect of the calcined tooth powder on autophagy mechanism of the in vitro mineralization of human periodontal ligament stem cells

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