TRAF6/c-fos在PTH加速兔下颌骨截骨术后正畸牙移动机制的初步研究

Abstract

Abstract: Objective　To investigate the expression changes of TRAF6 and c-fos mRNA on orthodontic tooth movement after mandibular ramus osteotomy regulated by parathyroid hormone (PTH）. Methods Forty eight rabbits were randomly divided into experimental group and control group. An experimental model of mandibular ramus osteotomy with installation of an orthodontic tooth movement device was established. The experimental group received intermittent subcutaneous injections of PTH 40 μg/kg and the control group received injections of normal saline postoperatively. The mesio movement speed of the first molars in the lower jaw was measured after surgery. The histomorphological changes on the compression side of orthodontic teeth was observed by HE staining. The number of osteoclasts on the compression side of periodontal tissue was counted by TRAP staining. Changes in expression of TRAF6 mRNA and c-fos mRNA in the periodontal tissues of the first molars were evaluated by quantitative fluorescence polymerase chain reaction. Results The movement of the first molars was more rapid in experimental group than in control group in the 21 days postoperatively. The number of osteoclasts on the compression side of orthodontic teeth in the experimental group was larger. Significantly higher TRAF6 and c-fos mRNA levels were detected on the compression side of the periodontal tissues in experimental group than in the control group. Conclusions Administration of PTH can increase TRAF6 and c-fos mRNA expression in the compressed periodontal tissues, thereby promoting maturity and differentiation of osteoclasts and accelerating orthodontic tooth movement after mandibular ramus osteotomy.

Key words: mandibular ramus osteotomy, orthodontic tooth movement, orthodontic tooth movement, tumor necrosis factor receptor-associated factors 6, c-fos

CLC Number:

R783.5

References 26

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Preliminary study on the mechanism of TRAF6/c-fos in PTH-accelerated orthodontic tooth movement after mandibular ramus osteotomy

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