Abstract: Objective To investigate the influence of CpG 2006 on tooth movement and alveolar bone remodeling in rats. Methods Male Wistar rats were chosen. Orthodontic tooth movement devices were installed at both maxillary of each rat, and the left side was chosen to be the treatment lateral, while the right side the control lateral. CpG 2006 or PBS was injected under the buccal mucosa of the maxillary first molar. Injection repeated every 72 h to all the animals sacrificed 14 days later. The migration distance of the molar was measured depending on specimen and Xray. The expression levels of osteoclast and inflammatory related factors were detected by Realtime PCR. HE staining was used for detecting the effects of CpG 2006 on the organ. Results The migration distance of the molar in treatment lateral was smaller and the expression levels of IL1,IL6,TNFɑ mRNA were lower than the control lateral, while the expression levels of Runx2,SP7,OCN mRNA were higher than the control lateral, and there were no differences among the expression levels of Nfatc,CTSK, MMP9 mRNA between the two laterals. No pathological changes were found in their organs. Conclusion CpG 2006 can regulate the distance of tooth movement by promoting the osteogenic activation of periodontal tissue, and reduce the expression of inflammatory factors during tooth movement.

Key words: CpG 2006, osteogenesis, inflammation, orthodontic tooth movement, rats