正畸骨改建中Hedgehog通路下游信号的表达变化

Abstract

Abstract: Abstract: Objective Hedgehog pathway play a vital role in bone development. This study investigated the expression of Hedgehog pathway signals in orthodontic bone remodeling. Methods We established the mice orthodontic model and observed on day 0, day 7, day 14 after orthodontic treatment. Tooth movement distance measured by micro-CT. HE&TRAP staining were used to investigated the orthodontic bone remodeling and osteoclastogenesis. Immunofluorescence staining was used for the expression of Gli1 positive cells in bone remodeling. Hedgehog pathway molecules mRNA expression levels and the expression changes of Intraflagellar transport(IFT) protein detected by Real time-PCR. Results The orthodontic tooth movement distance gradually increased. HE&TRAP staining showed the alveolar bone remodeling is obviously and osteoclastogenesis actively after treatment. Immunofluorescence showed a significant increased in the expression of Gli1 positive cells, which was mainly expressed in the alveolar bone pressure side, and peaked on the 7th day of orthodontic force(P<0.05). The mRNA expression of Hedgehog pathway vital molecules Shh, Smo, Gli were higher than control group(P<0.05). The changes of intraflagellar transport protein mRNA expression levels at each time point were statistically significant (P<0.05). Conclusion The Hedgehog pathway signals and Intraflagellar transport (IFT) protein participate in the orthodontic alveolar bone remolding.

Key words: Key words: orthodontic bone remodeling, osteoclast, hedgehog pathway, intraflagellar transport (IFT) protein

CLC Number:

R783.5

References 24

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The Expression of Hedgehog downstream signals in orthodontic bone remodeling.

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