Abstract: Abstract：　Objective The aim of this study is to examined the effect of T3 on the odonto/ostegenic differentiation of SCAPs. Methods After treated with T3, the ALP was measured to select the optimum concentration. The CCK‐8 were taken to distinguish the proliferation ability between the T3-treated group and the control group. To evaluate the odonto/ostegenic differentiation potential of the T3-treated SCAPs, RT-PCR, western blot, Alkaline Phosphatase Staining was performed. The MAPK signal pathway was detected via western blot. Results 10-9 mol/L T3-treated group expressed the highest ALP among all groups (p < 0.01) and it was chosen as the optimal concentration. CCK-8 demonstrated that T3 had no significant effect on the proliferation of SCAPs (p > 0.05). Moreover, results of real-time RT-PCR, western blot, showed that 10-9 mol/L T3 increased odonto/ostegenic differentiation of SCAPs. Besides, ERK signal pathway was activated at 15 min after treated with T3. The odonto/ostegenic related protein was suppressed by U0126. Conclusions 10-9 mol/L T3 promote the osteogenic differentiation of SCAPs and this process may be closely realted to the ERK signal pathway.

Key words: T3, SCAPs, odonto/ostegenic differentiation