人羊膜间充质干细胞旁分泌对成骨细胞的影响

Abstract

Abstract: Objective　To investigate the effect of human amniotic mesenchymal stem cells(hAMSCs) on osteoblast by paracrine manner. Methods　Primary hAMSCs were extracted by enzymatic digestion, and surface antigen markers were identified. Culture supernatant of hAMSCs after 24 h was collected and mixed with fresh medium by 1∶1, and was defined as condition medium(hAMSC-CM), while fresh medium was set as negative control. Their effects on the activities of MG63 were then detected. Cell proliferation was detected by MTT continuously for 4 days. The migrated cell number was estimated and counted after hematoxylin eosin staining by means of transwell system 2.5 h later. Alkaline phosphatase activity was measured after 5 days with p-Nitrophenyl Phosphate Disodium(pNPP). Results　hAMSCs showed the shape of fibroblast. Surface antigen markers were positive for CD29, CD105, CD49d, CD44， while negative for CD34, CD45, CD31, HLADR. hAMSC-CM promoted MG63 proliferation significantly during the first 3 days(P<0.05). Migration ability in hAMSC-CM group was enhanced prominently(P<0.05). ALP activity in cells cultured with hAMSC-CM exceeded control group markedly(P<0.05). Conclusion　hAMSCs are able to enhance the biological activity of osteoblast through CM mediated paracrine.

Key words: human amniotic mesenchymal stem cells, osteoblast, condition medium, paracrine

CLC Number:

R329.28

References 21

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Effect of human amniotic mesenchymal stem cells on osteoblast by paracrine

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