三维多孔支架表面成型二氧化钛纳米管阵列体外促进人牙髓干细胞黏附及成骨分化

Abstract

Abstract: Objective　 To fabricate titanium dioxide (TiO2) nanotube arrays on the surface of 3D porous zirconium dioxide（ZrO2）scaffolds and detect their influence on adhesion and proliferation of human dental pulp stem cells (hDPSCs). Methods　 ZrO2 porous scaffolds made by replication technique were coated with TiO2 slurry of 5%, 15%, 30% mass fractions respectively. After being heated for 2h at 500℃, samples were respectively immersed in 10mol/L NaOH for 12, 24, 36 h at 110℃. Compressive strength and porosity OF scaffolds were characterized, and SEM scanned their surface, while EDS examined their element types and proportions. Confirmed crystal structures of which by LRS, the experimental group（formed with best TiO2 nanotube arrays）and the control group (without TiO2 nanotube arrays) were seeded with hDPSCs. Cells’ adhesion was visualized by SEM and phalloidin, while their osteogenic differentiation was determined by ALP and proliferation was determined by CCK-8. Results Confirmed as rutile, TiO2 nanotube arrays were formed on the surface of the samples treated with 15% TiO2 slurry and NaOH for 24 hours. The compressive strength was 1.76 MPa, and the porosity was 79%. Compared with the control group, cells from the experimental group had more pseudopodia, extended better after being seeded for 6h and produced significantly higher ALP, while results of CCK-8 showed no statistical difference. Conclusions　 This experiment successfully forms TiO2 nanotube arrays on the surface of 3D porous ZrO2 scaffolds. The TiO2 nanotube arrays mildly strengthen scaffolds and facilitate adhesion and osteogenic differentiation of human dental pulp stem cells in vitro, but have no influence on proliferation.

Key words: titanium, nanotubes, bone-tissue-engineering, scaffold, dental pulp stem cells

CLC Number:

R783.1

References 22

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Titanium dioxide nanotube arrays formed on 3D porous scaffold facilitated adhesion and osteogenic differentiation of human dental pulp stem cells in vitro

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