Abstract: 【Abstract】 Objective To explore the feasibility of integrating GuttaFlow technique into Thermoplasticized Gutta-percha Obtura Ⅱ technique. Methods Five groups of extracted human teeth with single root(totally 70) were respectively obturated with Obtura Ⅱ( group A), Touch-heat+ Obtura Ⅱ(group B), GuttaFlow+ Obtura Ⅱ technique (group C), cold lateral condensation (group D) and nothing (groupE). After condensation, the coronal part of each root was immersed in 1mol/L glucose solution and the apical part in 1ml distilled water. Glucose solution was forced under a 15cm pressure toward the apical part of the root. Leakage along the root filling was measured by the concentration of glucose in apical reservoir 1, 2, 4, 7, 10, 15, 20, 25 and 30 days respectively after the treatment. Results The microleakage has continuously increased with the increase of time and there was no significant difference in the leakage among three test groups(A,B,C) after condensation(P＞0.05). Conclusions It is feasible to integrate GuttaFlow technique into Thermoplasticized Gutta-percha Obtura Ⅱ technique.